Dataset Information

CodY - The impact of CodY on virulence determinant production in community-associated methicillin-resistant Staphylococcus aureus

ABSTRACT: The Impact of CodY on Virulence Determinant Production in Community-Associated Methicillin Resistant Staphylococcus aureus

INSTRUMENT(S): instrument model, LTQ Orbitrap XL

ORGANISM(S): Staphylococcus Aureus Subsp. Aureus Usa300_fpr3757

SUBMITTER: Frances Rivera

PROVIDER: PRD000448 | Pride | 2012-05-17

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_17700.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_17700.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_17700.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_17701.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_17701.pride.mztab.gz	Mztab

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Publications

The impact of CodY on virulence determinant production in community-associated methicillin-resistant Staphylococcus aureus.

Rivera Frances E FE Miller Halie K HK Kolar Stacey L SL Stevens Stanley M SM Shaw Lindsey N LN

Proteomics 20111220 2

Staphylococcus aureus is a leading human pathogen of both hospital and community-associated diseases worldwide. This organism causes a wealth of infections within the human host as a result of the vast arsenal of toxins encoded within its genome. Previous transcriptomic studies have shown that toxin production in S. aureus can be strongly impacted by the negative regulator CodY. CodY acts by directly, and indirectly (via Agr), repressing toxin production during times of plentiful nutrition. In t ...[more]

PMID: 22106056

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Project description:Approximatively 75% of the genome of S. aureus (“core” genome) are highly conserved between strains, whereas the remaining 25% (“accessory” genome) are composed of variable regions that are mostly composed of mobile genetic elements (MGE), containing virulence and resistance genes. We have developed a composite DNA-microarray (StaphVar Array) which selectively targets 403 genes located on the accessory or core variable genome. Target genes encode antimicrobial resistance factors (35 %), virulence factors (28 %) and adhesins (31%). This microarray was validated with reference strains and used to characterize the genomic DNA of 13 community-acquired methicillin resistant S. aureus (CA-MRSA) strains representative of all the Multilocus Sequence Types (STs) described to date in Belgium. Analysis of gene content of 8 reference strains by the StaphVar Array matched 96 to 99% of the theoretical results. Analysis of CA-MRSA strains showed that 54.4 % of the genes tested were strain-dependent. Strains presented specific exotoxin, enterotoxin, cytolysin and adhesin gene profile by multi-locus sequence typing (MLST) lineage. One exception to these “lineage-specific” gene profile was the variable presence of the Arginin Catabolic Mobile Element (ACME, characteristic of the USA300 clone) within ST8 strains. In conclusion, this novel StaphVar array enables characterization of more than 400 variable resistance and virulence determinants in S. aureus strains. CA-MRSA strains from Belgium displayed extensive diversity in virulence and resistance profile. The presence of the USA 300 clone in our country was confirmed. Although mainly located on MGE, association of virulence genes were highly conserved within strains of the same MLST lineage. CGH microarray was performed on 13 epidemiologically distinct clinical isolates of methicillin resistant S. aureus. S. aureus labeled genomic DNA were hybridized to StaphVar arrays containing 1326 60mer oligonucleotide probes (Eurogentec, Belgium).