Name: Comparative analysis of five different preparation methods for identification of complex protein mixtures in yeast
Keywords: Not available

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Comparative analysis of five different preparation methods for identification of complex protein mixtures in yeast

ABSTRACT: Not available

INSTRUMENT(S): instrument model

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Attila Csordas

PROVIDER: PRD000460 | Pride | 2012-07-26

REPOSITORIES: Pride

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Comprehensive proteomics in yeast using chromatographic fractionation, gas phase fractionation, protein gel electrophoresis, and isoelectric focusing.

Breci Linda L Hattrup Emily E Keeler Matthew M Letarte Jessica J Johnson Roxanne R Haynes Paul A PA

Proteomics 20050501 8

We have investigated the use of a variety of different techniques to identify as many proteins as possible in a yeast lysate, with the aim of investigating the overlap and complementarity of data from different approaches. A standard lysate was prepared from log phase yeast (Saccharomyces cerevisiae). This was then subjected to analysis via five different approaches aimed at identifying as many proteins as possible using an ion trap mass spectrometer. The total number of non-redundant protein id ...[more]

PMID: 15852344

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