Integrated genomic and proteomic analyses of a systematically perturbed metabolic network
Ontology highlight
ABSTRACT:
INSTRUMENT(S): instrument model
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)
SUBMITTER: 
Attila Csordas
PROVIDER: PRD000468 | Pride | 2012-07-26
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| PRIDE_Exp_Complete_Ac_17929.pride.mgf.gz | Mgf | |||
| PRIDE_Exp_Complete_Ac_17929.pride.mztab.gz | Mztab | |||
| PRIDE_Exp_Complete_Ac_17929.xml.gz | Xml |
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Publications
Integrated genomic and proteomic analyses of a systematically perturbed metabolic network.
Ideker T T Thorsson V V Ranish J A JA Christmas R R Buhler J J Eng J K JK Bumgarner R R Goodlett D R DR Aebersold R R Hood L L
Science (New York, N.Y.) 20010501 5518
We demonstrate an integrated approach to build, test, and refine a model of a cellular pathway, in which perturbations to critical pathway components are analyzed using DNA microarrays, quantitative proteomics, and databases of known physical interactions. Using this approach, we identify 997 messenger RNAs responding to 20 systematic perturbations of the yeast galactose-utilization pathway, provide evidence that approximately 15 of 289 detected proteins are regulated posttranscriptionally, and ...[more]
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