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Quantitative analysis of human plasma in vivo lipopolysaccharide administration using ¹?O/¹?O Labeling and AMT

ABSTRACT: Not available

INSTRUMENT(S): Fourier Transform Ion Cyclotron Resonance Mass Spectrometer, instrument model

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Attila Csordas

PROVIDER: PRD000478 | Pride | 2012-07-26

REPOSITORIES: Pride

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			Action	DRS
	PRIDE_Exp_Complete_Ac_18083.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_18083.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_18083.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_18084.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_18084.pride.mztab.gz	Mztab

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Publications

Quantitative proteome analysis of human plasma following in vivo lipopolysaccharide administration using 16O/18O labeling and the accurate mass and time tag approach.

Qian Wei-Jun WJ Monroe Matthew E ME Liu Tao T Jacobs Jon M JM Anderson Gordon A GA Shen Yufeng Y Moore Ronald J RJ Anderson David J DJ Zhang Rui R Calvano Steve E SE Lowry Stephen F SF Xiao Wenzhong W Moldawer Lyle L LL Davis Ronald W RW Tompkins Ronald G RG Camp David G DG Smith Richard D RD

Molecular & cellular proteomics : MCP 20050307 5

Identification of novel diagnostic or therapeutic biomarkers from human blood plasma would benefit significantly from quantitative measurements of the proteome constituents over a range of physiological conditions. Herein we describe an initial demonstration of proteome-wide quantitative analysis of human plasma. The approach utilizes postdigestion trypsin-catalyzed 16O/18O peptide labeling, two-dimensional LC-FTICR mass spectrometry, and the accurate mass and time (AMT) tag strategy to identify ...[more]

PMID: 15753121

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Quantitative proteome analysis of human plasma following in vivo lipopolysaccharide administration using 16O/18O labeling and the accurate mass and time tag approach.

Project description:Identification of novel diagnostic or therapeutic biomarkers from human blood plasma would benefit significantly from quantitative measurements of the proteome constituents over a range of physiological conditions. Herein we describe an initial demonstration of proteome-wide quantitative analysis of human plasma. The approach utilizes postdigestion trypsin-catalyzed 16O/18O peptide labeling, two-dimensional LC-FTICR mass spectrometry, and the accurate mass and time (AMT) tag strategy to identify and quantify peptides/proteins from complex samples. A peptide accurate mass and LC elution time AMT tag data base was initially generated using MS/MS following extensive multidimensional LC separations to provide the basis for subsequent peptide identifications. The AMT tag data base contains >8,000 putative identified peptides, providing 938 confident plasma protein identifications. The quantitative approach was applied without depletion of high abundance proteins for comparative analyses of plasma samples from an individual prior to and 9 h after lipopolysaccharide (LPS) administration. Accurate quantification of changes in protein abundance was demonstrated by both 1:1 labeling of control plasma and the comparison between the plasma samples following LPS administration. A total of 429 distinct plasma proteins were quantified from the comparative analyses, and the protein abundances for 25 proteins, including several known inflammatory response mediators, were observed to change significantly following LPS administration.

| S-EPMC1829297 | biostudies-literature

Quantitative proteome analysis of human blister fluids using 18O labeling