Dataset Information

Proteome dynamics of Streptomyces coelicolor during batch fermentation

ABSTRACT: Total protein S. coelicolor INB201 23h post inoculation (A)

OTHER RELATED OMICS DATASETS IN: PRJNA144841

INSTRUMENT(S): Bruker Daltonics HCT Series, instrument model

ORGANISM(S): Streptomyces Coelicolor

SUBMITTER: Louise Thomas

PROVIDER: PRD000514 | Pride | 2012-05-02

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_18302.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_18302.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_18302.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_18303.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_18303.pride.mztab.gz	Mztab

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Publications

Metabolic switches and adaptations deduced from the proteomes of Streptomyces coelicolor wild type and phoP mutant grown in batch culture.

Thomas Louise L Hodgson David A DA Wentzel Alexander A Nieselt Kay K Ellingsen Trond E TE Moore Jonathan J Morrissey Edward R ER Legaie Roxane R Wohlleben Wolfgang W Rodríguez-García Antonio A Martín Juan F JF Burroughs Nigel J NJ Wellington Elizabeth M H EM Smith Margaret C M MC

Molecular & cellular proteomics : MCP 20111206 2

Bacteria in the genus Streptomyces are soil-dwelling oligotrophs and important producers of secondary metabolites. Previously, we showed that global messenger RNA expression was subject to a series of metabolic and regulatory switches during the lifetime of a fermentor batch culture of Streptomyces coelicolor M145. Here we analyze the proteome from eight time points from the same fermentor culture and, because phosphate availability is an important regulator of secondary metabolite production, c ...[more]

PMID: 22147733

Similar Datasets

Project description:BackgroundSystems biology approaches to study metabolic switching in Streptomyces coelicolor A3(2) depend on cultivation conditions ensuring high reproducibility and distinct phases of culture growth and secondary metabolite production. In addition, biomass concentrations must be sufficiently high to allow for extensive time-series sampling before occurrence of a given nutrient depletion for transition triggering. The present study describes for the first time the development of a dedicated optimized submerged batch fermentation strategy as the basis for highly time-resolved systems biology studies of metabolic switching in S. coelicolor A3(2).ResultsBy a step-wise approach, cultivation conditions and two fully defined cultivation media were developed and evaluated using strain M145 of S. coelicolor A3(2), providing a high degree of cultivation reproducibility and enabling reliable studies of the effect of phosphate depletion and L-glutamate depletion on the metabolic transition to antibiotic production phase. Interestingly, both of the two carbon sources provided, D-glucose and L-glutamate, were found to be necessary in order to maintain high growth rates and prevent secondary metabolite production before nutrient depletion. Comparative analysis of batch cultivations with (i) both L-glutamate and D-glucose in excess, (ii) L-glutamate depletion and D-glucose in excess, (iii) L-glutamate as the sole source of carbon and (iv) D-glucose as the sole source of carbon, reveal a complex interplay of the two carbon sources in the bacterium's central carbon metabolism.ConclusionsThe present study presents for the first time a dedicated cultivation strategy fulfilling the requirements for systems biology studies of metabolic switching in S. coelicolor A3(2). Key results from labelling and cultivation experiments on either or both of the two carbon sources provided indicate that in the presence of D-glucose, L-glutamate was the preferred carbon source, while D-glucose alone appeared incapable of maintaining culture growth, likely due to a metabolic bottleneck at the oxidation of pyruvate to acetyl-CoA.