Dataset Information

HAPI - Proteomic analysis of rat microglia establishes a high-confidence reference data set of over 3000 proteins

ABSTRACT: Offline SCX fractionation coupled with in-line reverse phase LC and Orbitrap Mass Spectrometric analysis reveals 3,006 protein groups from the HAPI rat microglial cell line at an FDR of 1% for peptides and proteins.

INSTRUMENT(S): instrument model, LTQ Orbitrap XL

ORGANISM(S): Rattus Norvegicus (rat) Glia

SUBMITTER: Harris Bell-Temin

PROVIDER: PRD000518 | Pride | 2013-07-10

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	PRIDE_Exp_Complete_Ac_18380.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_18380.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_18381.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_18381.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_18382.pride.mgf.gz	Mgf

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Proteomic analysis of rat microglia establishes a high-confidence reference data set of over 3000 proteins.

Bell-Temin Harris H Barber David S DS Zhang Ping P Liu Bin B Stevens Stanley M SM

Proteomics 20111220 2

Highly aggressively proliferating immortalized (HAPI) microglial cells have been used as an in vitro model for investigating key microglial functions including inflammatory, neurotoxic, and phagocytic activities. Through the use of offline strong cation-exchange fractionation followed by inline reversed-phase chromatographic separation and tandem mass spectrometric analysis on a hybrid linear ion trap-Orbitrap instrument, the HAPI microglial proteome was characterized to a depth of 3006 unique p ...[more]

PMID: 22121004

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