INS-1 832/13 lipid droplet proteome
Ontology highlight
ABSTRACT:
INSTRUMENT(S): instrument model
ORGANISM(S): Rattus Norvegicus (rat)
SUBMITTER: 
Svante Resjo
PROVIDER: PRD000592 | Pride | 2012-01-19
REPOSITORIES: Pride
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Publications
Characterization of the lipid droplet proteome of a clonal insulin-producing β-cell line (INS-1 832/13).
Larsson Sara S Resjö Svante S Gomez Maria F MF James Peter P Holm Cecilia C
Journal of proteome research 20120124 2
Lipids are known to play a crucial role both in the normal control of insulin release and in the deterioration of β-cell function, as observed in type 2 diabetes. Despite this established dual role of lipids, little is known about lipid storage and handling in β-cells. Here, we isolated lipid droplets from oleate-incubated INS-1 832/13 cells and characterized the lipid droplet proteome. In a total of four rounds of droplet isolation and proteomic analysis by HPLC-MS/MS, we identified 96 proteins ...[more]
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