Proteomics

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Survey of the camel urinary proteome


ABSTRACT: Proteins extracted from camel urine by ultrafiltration and precipitation were fractionated by SDS-PAGE, digested in-gel, then analysed by LC-MS/MS with camel EST sequence database search

INSTRUMENT(S): Bruker Daltonics HCT Series, instrument model

ORGANISM(S): Camelus Dromedarius (dromedary) (arabian Camel)

TISSUE(S): Urine

SUBMITTER: David Stead  

PROVIDER: PRD000616 | Pride | 2012-09-26

REPOSITORIES: Pride

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Survey of the camel urinary proteome by shotgun proteomics using a multiple database search strategy.

Alhaider Abdulqader A AA   Bayoumy Nervana N   Argo Evelyn E   Gader Abdel G M A AG   Stead David A DA  

Proteomics 20121019 22


We report the first survey of the dromedary camel urinary proteome. Proteins retained from ultrafiltration of urine were analysed by GeLC-MS/MS (SDS-PAGE followed by LC-MS/MS). In the absence of a complete camel genome sequence, the number of protein identifications was maximised by searching three primary sequence databases: Swiss-Prot, alpaca and camel EST. This search strategy enabled the identification of 1274 peptide sequences, of which 735 were found in at least two independent samples. Fu  ...[more]

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