Dataset Information

Investigation of zinc hyperaccumulation in Thlaspi caerulescens using a quantitative proteomics approach

ABSTRACT: Investigation of zinc hyperaccumulation in Thlaspi caerulescens using a quantitative proteomics approach

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Thlaspi Caerulescens (alpine Penny-cress) (thlaspi Calaminare)

TISSUE(S): Mesophyll, Leaf, Lower Epidermis

SUBMITTER: Thomas Schneider

PROVIDER: PRD000636 | Pride | 2014-03-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_21354.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_21354.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_21354.xml.gz	Xml

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Publications

A proteomics approach to investigate the process of Zn hyperaccumulation in Noccaea caerulescens (J & C. Presl) F.K. Meyer.

Schneider Thomas T Persson Daniel Pergament DP Husted Søren S Schellenberg Maja M Gehrig Peter P Lee Youngsook Y Martinoia Enrico E Schjoerring Jan K JK Meyer Stefan S

The Plant journal : for cell and molecular biology 20121026 1

Zinc (Zn) is an essential trace element in all living organisms, but is toxic in excess. Several plant species are able to accumulate Zn at extraordinarily high concentrations in the leaf epidermis without showing any toxicity symptoms. However, the molecular mechanisms of this phenomenon are still poorly understood. A state-of-the-art quantitative 2D liquid chromatography/tandem mass spectrometry (2D-LC-MS/MS) proteomics approach was used to investigate the abundance of proteins involved in Zn ...[more]

PMID: 22974502

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Project description:This a model from the article: The dynamic balance of import and export of zinc in Escherichia coli suggests a heterogeneous population response to stress Takahashi H, Oshima T, Doherty N, Clayton SR, Iqbal M, Hill PJ, Hobman JL, Tobe T, Ogasawara N, Kanaya S and Stekel DJ journal year id, Abstract: Zinc is essential for life, but toxic in excess. Thus all cells must control their internal zinc concentration. We used a systems approach, alternating rounds of experiments and models, to further elucidate the zinc control systems in Escherichia coli. We measured the response to zinc of the main specific zinc import and export systems in the wild type, and a series of deletion mutant strains. We interpreted these data with a detailed mathematical model and Bayesian model fitting routines. There are three key findings: First, that alternate, non-inducible importers and exporters are important. Second, that an internal zinc reservoir is essential for maintaining the internal zinc concentration. Third, our data fitting led us to propose that the cells mount a heterogeneous response to zinc: some respond effectively, while others die or stop growing. In a further round of experiments we demonstrated lower viable cell counts in the mutant strain tested exposed to excess zinc, consistent with this hypothesis. A stochastic model simulation demonstrated considerable fluctuations in the cellular levels of the ZntA exporter protein, reinforcing this proposal. We hypothesise that maintaining population heterogeneity could be a bet-hedging response allowing a population of cells to survive in varied and fluctuating environments. This model originates from BioModels Database: A Database of Annotated Published Models (http://www.ebi.ac.uk/biomodels/). It is copyright (c) 2005-2011 The BioModels.net Team. For more information see the terms of use. To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le NovÃ¨re N, Laibe C (2010) BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC Syst Biol., 4:92.