Proteomics

Dataset Information

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Human MNC proteome - Proteome profiling of peripheral mononuclear cells from human blood


ABSTRACT: Proteome characterization of mononuclear cells extracted from human blood - Dataset 3

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Mononuclear Cell, Blood

SUBMITTER: Daniel Martins-de-Souza  

PROVIDER: PRD000732 | Pride | 2013-01-03

REPOSITORIES: Pride

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Publications

Proteome profiling of peripheral mononuclear cells from human blood.

Maccarrone Giuseppina G   Rewerts Christiane C   Lebar Maria M   Turck Christoph W CW   Martins-de-Souza Daniel D  

Proteomics 20130124 5


Peripheral blood mononuclear cells (MNCs) are accessible through blood collection and represent a useful source for investigations on disease mechanisms and treatment response. Aiming to build a reference proteome database, we generated three proteome data sets from MNCs using a combination of SDS-PAGE and nanoflow LC-MS. Experiments were performed in triplicates and 514 unique proteins were identified by at least two non-redundant peptides with 95% confidence for all replicates. Identified prot  ...[more]

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