Proteomics

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Cancer-derived myofibroblasts vs. non-cancer derived myofibroblasts

ABSTRACT: All samples are conditioned media from primary cultured human gastric myofibroblasts, derived from either carcinoma tissue, or adjacent non cancerous tissue. In each experiment the cancer-derived myofibroblasts are compared against the non-cancer derived myofibroblasts from the same patient. All of the experiments were samples from Patient 1, except for 648, which is from Patient 2. SILAC labelling in each case is; 567 - Methionine COFRADIC; Cancer-derived = Heavy, Non-cancer = Light 575 - N-terminal COFRADIC; Cancer-derived = Heavy, Non-cancer = Light 646 - Cancer-derived = Light, Non-cancer = Heavy 647 - Non-cancer = Heavy, Non-cancer = Light (same cell line labelled twice to create a control experiment) 648 - Cancer-derived = Heavy, Non-cancer = Light

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Pieter-Jan De Bock  

PROVIDER: PRD000744 | Pride | 2013-06-20

REPOSITORIES: Pride

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Dataset's files

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Action DRS
PRIDE_Exp_Complete_Ac_27157.pride.mgf.gz Mgf
PRIDE_Exp_Complete_Ac_27157.pride.mztab.gz Mztab
PRIDE_Exp_Complete_Ac_27157.xml.gz Xml
PRIDE_Exp_Complete_Ac_27158.pride.mgf.gz Mgf
PRIDE_Exp_Complete_Ac_27158.pride.mztab.gz Mztab
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Publications

Mapping proteolytic processing in the secretome of gastric cancer-associated myofibroblasts reveals activation of MMP-1, MMP-2, and MMP-3.

Holmberg Christopher C   Ghesquière Bart B   Impens Francis F   Gevaert Kris K   Kumar J Dinesh JD   Cash Nicole N   Kandola Sandhir S   Hegyi Peter P   Wang Timothy C TC   Dockray Graham J GJ   Varro Andrea A  

Journal of proteome research 20130605 7

Cancer progression involves changes in extracellular proteolysis, but the contribution of stromal cell secretomes to the cancer degradome remains uncertain. We have now defined the secretome of a specific stromal cell type, the myofibroblast, in gastric cancer and its modification by proteolysis. SILAC labeling and COFRADIC isolation of methionine containing peptides allowed us to quantify differences in gastric cancer-derived myofibroblasts compared with myofibroblasts from adjacent tissue, rev  ...[more]

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