Dataset Information

Global liver proteomics of rats exposed for five days to phenobarbital identifies changes associated with cancer and with cyp metabolism

ABSTRACT: Adult male Sprague Dawley-derived rats were housed and fed as described previously (Dail et al., 2007). Three rats were treated i.p. with PB in saline at 80 mg/kg body weight/day (treated) for 5 days as described previously (Ma and Chambers; 1995). Following sacrifice on day 5, the livers were removed and stored at -80 C as previously described (Dail et al., 2007). Five 8 µm sections were taken from each of the three control and three experimental livers resulting in fifteen sections from each group using a Triangle Biomedical Supply Co Minotome Plus (Durham, NC) microtome. Differential detergent fractionation two dimensional liquid chromatography electrospray ionization tandem mass spectrometry (DDF 2D LC ESI MS2) and systems biology modeling were used to identify alterations in toxicologically relevant hepatic molecular functions and biological processes in the livers of rats

INSTRUMENT(S): instrument model, LCQ Deca XP Plus

ORGANISM(S): Rattus Norvegicus (rat)

SUBMITTER: Shane Burgess

PROVIDER: PRD000797 | Pride | 2008-12-09

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	PRIDE_Exp_Complete_Ac_7970.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_7970.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_7971.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_7971.xml.gz	Xml

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Publications

Spatial distribution of CYP2B1/2 messenger RNA within the rat liver acinus following exposure to the inducers phenobarbital and dieldrin.

Dail Mary B MB Burgess Shane C SC Meek Edward C EC Wagner Jennifer J Baravik Jeffrey J Chambers Janice E JE

Toxicological sciences : an official journal of the Society of Toxicology 20070521 1

Traditionally, the liver has been considered a homogeneous organ, but literature suggests that the cytochromes P450 are differentially distributed among the hepatocytes and that the pattern of this distribution is altered by various xenobiotics. In this study, the CYP2B1/2 messenger RNA (mRNA) in the hepatocytes was compared following treatment of rats with either of two inducers, phenobarbital (PB), or dieldrin. Adult male Sprague-Dawley-derived rats were treated with either ip PB in saline at ...[more]

PMID: 17517822

Publication: 1/2

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