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Cell-specific information processing in segregating populations of Eph receptor-ephrin-expressing cells

ABSTRACT: Cell-specific information processing in segregating populations of Eph receptor-ephrin-expressing cells

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

SUBMITTER: Claus Jorgensen

PROVIDER: PRD000814 | Pride | 2009-12-11

REPOSITORIES: Pride

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			Action	DRS
	PRIDE_Exp_Complete_Ac_10295.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_10295.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_10295.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_10296.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_10296.pride.mztab.gz	Mztab

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Publications

Cell-specific information processing in segregating populations of Eph receptor ephrin-expressing cells.

Jørgensen Claus C Sherman Andrew A Chen Ginny I GI Pasculescu Adrian A Poliakov Alexei A Hsiung Marilyn M Larsen Brett B Wilkinson David G DG Linding Rune R Pawson Tony T

Science (New York, N.Y.) 20091201 5959

Cells have self-organizing properties that control their behavior in complex tissues. Contact between cells expressing either B-type Eph receptors or their transmembrane ephrin ligands initiates bidirectional signals that regulate cell positioning. However, simultaneously investigating how information is processed in two interacting cell types remains a challenge. We implemented a proteomic strategy to systematically determine cell-specific signaling networks underlying EphB2- and ephrin-B1-cont ...[more]

PMID: 20007894

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Project description:The goal of this study was to identify immediate early transcriptional targets of ephrin-B1 forward signaling that are relevant to palatogenesis. The Ephs compose a family of receptor tyrosine kinase signaling molecules that can be activated by their cognate ligands, the ephrins. Despite the importance of Eph/ephrin signaling in a wide variety of developmental and cell biological processes, a potential downstream transcriptional response has not been explored. To understand the role of ephrin-B1 signaling in palatogenesis, we examined transcriptional response to ephrin-B1 in a primary mouse embryonic palatal shelf cell culture system. We find an immediate early signature of gene expression that reflects the activation of Erk/MAPK signaling by ephrin-B1 signaling in the palatal shelves. Induction of primary palate cells with 2 ug/ml of pre-clustered ephrin-B1 for one hour. Uninduced primary palate cells are the control. Four replicates each.

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