Dataset Information

DK0025,30,31 - Alterations in the proteome of the respiratory tract in response to single and multiple exposures to naphthalene

ABSTRACT: Collaboration with Alan Buckpitt and Laura VanWinkle

INSTRUMENT(S): Bruker Daltonics micrOTOF series, instrument model

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Nasal Epithelium, Airway Epithelium

SUBMITTER: Dietmar Kültz

PROVIDER: PRD000846 | Pride | 2015-04-17

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_30584.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_30584.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_30584.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_30585.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_30585.pride.mztab.gz	Mztab

Items per page:

1 - 5 of 6

Publications

Alterations in the proteome of the respiratory tract in response to single and multiple exposures to naphthalene.

Kültz Dietmar D Li Johnathon J Sacchi Romina R Morin Dexter D Buckpitt Alan A Van Winkle Laura L

Proteomics 20150513 15

Protein adduction is considered to be critical to the loss of cellular homeostasis associated with environmental chemicals undergoing metabolic activation. Despite considerable effort, our understanding of the key proteins mediating the pathologic consequences from protein modification by electrophiles is incomplete. This work focused on naphthalene (NA) induced acute injury of respiratory epithelial cells and tolerance which arises after multiple toxicant doses to define the initial cellular pr ...[more]

PMID: 25825134

Similar Datasets

Project description:We previously reported that bacterial diversity in sputum samples from never-smoking women in rural China varied by lung cancer status and household air pollution (HAP) exposure type. Here, we expand on our associations between environmental exposures and respiratory tract microbiota with an additional 90 never-smoking women from Xuanwei, China. DNA from sputum samples of cases (n = 45) and controls (n = 45) was extracted using a multistep enzymatic and physical lysis, followed by a standardized clean up. V1-V2 regions of 16S rRNA genes were Polymerase chain reaction (PCR) amplified. Purified amplicons were sequenced by 454 FLX Titanium pyrosequencing and high-quality sequences were evaluated for diversity and taxonomic membership. In our population of never-smokers, increased risk of lung cancer was associated with lower alpha diversity compared to higher alpha diversity (Shannon: ORhigh = 1.00 [reference], ORmedium = 3.84 [1.02-14.48], ORlow = 3.78 [1.03-13.82]; observed species: ORhigh = 1.00 [reference], ORmedium = 2.37 [0.67-8.48], ORlow = 2.01 [0.58-6.97]; Phylogenetic Diversity (PD) whole tree: ORhigh = 1.00 [reference], ORmedium = 3.04 [0.85-10.92], ORlow = 2.53 [0.72-8.96]), as well as a decreased relative abundance of Fusobacteria (ORhigh = 1.00 [reference], ORmedium = 1.24 [0.42-3.66], ORlow = 2.01 [0.63-6.44], ptrend = 0.03). Increasing alpha diversity was associated with smoky coal use compared to clean fuel use among all subjects (observed species, P = 0.001; PD whole tree, P = 0.006; Shannon, P = 0.0002), as well as cases (observed species, P = 0.02; PD whole tree, P = 0.03; Shannon, P = 0.03) and controls (observed species, P = 0.01; PD whole tree, P = 0.05; Shannon, P = 0.002). Increased diversity was also associated with presence of livestock (observed species, P = 0.02; PD whole tree, P = 0.02; Shannon, P = 0.03) in the home for cases. Our study is the first to report that decreased microbial diversity is associated with risk of lung cancer. Larger studies are necessary to elucidate the direct and indirect effects attributed to the disease-specific, HAP-specific, and animal-specific associations. Environ. Mol. Mutagen. 2019. © 2019 Wiley Periodicals, Inc.