Proteomics

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Human 293 cells PEDF LC-MS/MS - Adenoviral E4 gene stimulates secretion of pigmental epithelium derived factor (PEDF) that maintains long-term survival of human glomerulus-derived endothelial cells

ABSTRACT: Experiment was performed on 293 cells, details are presented in publication: Adenoviral E4 gene stimulates secretion of PEDF that maintains long-term survival of human glomerulus-derived endothelial cells Marina Jerebtsova‡§*, Namita Kumari‖, Yuri Obuhkov‖, and Sergei Nekhai‖†* (to be published in Molecular and Cellular Proteomics), please see more details in paper Workflow HPLC on PicoFrit C18 column, gradient 60 min from 2% to 30% ACN Nanospray 2 kV, flow rate 300 nL/min LTQ Orbitrap MS. Data dependent scan FT MS + 3 FT MS/MS. A blank water run was performed after each sample run Each 24 hours setup performance was tested on Apomyoglobin sample, 12 fmol. Coverage was 39-79 % Search is made by SEQUEST on human database

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yuri Obukhov  

LAB HEAD: Yuri Obukhov

PROVIDER: PXD000014 | Pride | 2012-08-08

REPOSITORIES: Pride

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Adenoviral E4 gene stimulates secretion of pigmental epithelium derived factor (PEDF) that maintains long-term survival of human glomerulus-derived endothelial cells.

Jerebtsova Marina M   Kumari Namita N   Obuhkov Yuri Y   Nekhai Sergei S  

Molecular & cellular proteomics : MCP 20120821 11

Renal glomerular endothelial cells are specialized cells with an important role in physiological filtration and glomerular disease. However, maintenance of human primary endothelial cells requires stimulation with serum and growth factors that often results in modification of the cells properties. Previously, expression of early adenovirus region E4 was shown to help maintaining long-term survival of human endothelial cells in serum free media without addition of growth factors. In the current s  ...[more]

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