Dataset Information

D-score - D-score: a search engine independent MD-score

ABSTRACT: Processing of the dataset of synthetic phosphopeptides by Savitzki et al. (MCP, 2011) using multiple search engines. Establishment of the D-score: a search engine independent MD-score.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Marc Vaudel

LAB HEAD: Marc Vaudel

PROVIDER: PXD000021 | Pride | 2013-01-23

REPOSITORIES: Pride

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D-score: a search engine independent MD-score.

Vaudel Marc M Breiter Daniela D Beck Florian F Rahnenführer Jörg J Martens Lennart L Zahedi René P RP

Proteomics 20130215 6

While peptides carrying PTMs are routinely identified in gel-free MS, the localization of the PTMs onto the peptide sequences remains challenging. Search engine scores of secondary peptide matches have been used in different approaches in order to infer the quality of site inference, by penalizing the localization whenever the search engine similarly scored two candidate peptides with different site assignments. In the present work, we show how the estimation of posterior error probabilities for ...[more]

PMID: 23307401

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