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EHEC O157:H7 Sakai vs. TW14359: Secreted and Cytosolic iTRAQ; sepD secretome analysis of TW14359

ABSTRACT: Comparative analysis of EHEC O157:H7 Sakai vs. TW14359 secreted and cytosolic protein using iTRAQ and mass spectrometry on an LTQ Orbitrap. Samples were fractionated through strong cation exchange and HPLC before analsyis. For TW14359 sepD secretome analysis, strains were similarly prepared and analyzed. All samples were searched against a TW14359 specific protein database (Uniprot) coupled with randomized protein sequences.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Escherichia Coli O157:h7 (strain Tw14359 / Ehec)

SUBMITTER: Jason Morgan

LAB HEAD: Jason Morgan

PROVIDER: PXD000023 | Pride | 2013-09-05

REPOSITORIES: Pride

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			Action	DRS
	2012-03-02-SepD-01.RAW	Raw
	2012-03-02-SepD-02.RAW	Raw
	2012-03-02-SepD-03.RAW	Raw
	2012-03-02-SepD-04.RAW	Raw
	2012-03-02-SepD-05.RAW	Raw

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RcsB determines the locus of enterocyte effacement (LEE) expression and adherence phenotype of Escherichia coli O157 : H7 spinach outbreak strain TW14359 and coordinates bicarbonate-dependent LEE activation with repression of motility.

Morgan Jason K JK Vendura Khoury W KW Stevens Stanley M SM Riordan James T JT

Microbiology (Reading, England) 20130828 Pt 11

The 2006 US spinach outbreak of Escherichia coli O157 : H7, characterized by unusually severe disease, has been attributed to a strain (TW14359) with enhanced pathogenic potential, including elevated virulence gene expression, robust adherence and the presence of novel virulence factors. This study proposes a mechanism for the unique virulence expression and adherence phenotype of this strain, and further expands the role for regulator RcsB in control of the E. coli locus of enterocyte effacemen ...[more]

PMID: 23985143

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