Proteomics

Dataset Information

0

Lotus japonicus phosphopeptide


ABSTRACT: Phosphopeptides were purified using Titansphere. Before MS analysis, phosphopeptides were desalted using StageTips with C18 Empore disk membranes. Phosphopeptides were analysed by Orbitrap MS.The obtained MS and MS/MS spectra data were searched against Kazusa DNA Research Institute published lotus EST database using the MASCOT program (FDR<1%).

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Lotus Japonicus

SUBMITTER: Yoko Ino  

LAB HEAD: Yoko Ino

PROVIDER: PXD000053 | Pride | 2013-10-29

REPOSITORIES: Pride

Dataset's files

Source:
altmetric image

Publications

Phosphoproteome analysis of Lotus japonicus seeds.

Ino Yoko Y   Ishikawa Akiyo A   Nomura Ayako A   Kajiwara Hideyuki H   Harada Kyuya K   Hirano Hisashi H  

Proteomics 20140101 1


In this study, we report the first dataset of phosphoproteins of the seeds of a model plant, Lotus japonicus. This dataset might be useful in studying the regulatory mechanisms of seed germination in legume plants. By proteomic analysis of seeds following water absorption, we identified a total of 721 phosphopeptides derived from 343 phosphoproteins in cotyledons, and 931 phosphopeptides from 473 phosphoproteins in hypocotyls. Kinase-specific prediction analyses revealed that different kinases w  ...[more]

Similar Datasets

| PRJNA13205 | ENA
2016-12-01 | GSE73069 | GEO
2011-09-16 | GSE31739 | GEO
2014-03-25 | E-MTAB-2418 | biostudies-arrayexpress
2017-06-01 | GSE78767 | GEO
2011-09-15 | E-GEOD-31739 | biostudies-arrayexpress
2013-11-01 | E-MTAB-2000 | biostudies-arrayexpress
| PRJNA1039326 | ENA
2023-05-22 | MSV000092000 | MassIVE
| PRJNA843370 | ENA