Proteomics

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An extensive proteome map of tomato (Solanum lycopersicum) fruit pericarp

ABSTRACT: Fruit pericarp at two stages, cell expasion and orange red. From Solaunm Lycopersicum and Solaunm Lycopersicum var cerasiforme genotypes. Two Dimensional Polyacrylamide Gel Electrophoresis (Coomasie staining, Band excision, Enzyme digestion) LC/MS (Thermo Finnigan LTQ XL) Software processing (X! Tandem, CYCLONE (2010.12.01.1)) Databases (Contaminants database is a home-made database. It is a set of 25 proteic sequences corresponding to proteins that could be present in the samples, 21 correspond with keratins, 2 with trypsin and 2 with bovin serum albumins. 17 sequences come from SwissProt and 8 from TrEMBL. ITAG2.3 database is the set of proteic sequences predicted in the version 2.3 of the annotation of the tomate genome published by the ITAG group (ftp.solgenomics.net))

INSTRUMENT(S): LTQ

ORGANISM(S): Solanum Lycopersicum

SUBMITTER: Laura Pascual  

PROVIDER: PXD000105 | Pride | 2013-09-11

REPOSITORIES: Pride

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20100824_Faurobert_026_19_D02_RA36.mzXML Mzxml
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An extensive proteome map of tomato (Solanum lycopersicum) fruit pericarp.

Xu Jiaxin J   Pascual Laura L   Aurand Rémy R   Bouchet Jean-Paul JP   Valot Benoît B   Zivy Michel M   Causse Mathilde M   Faurobert Mireille M  

Proteomics 20130913 20

Tomato (Solanum lycopersicum) is the model species for studying fleshy fruit development. An extensive proteome map of the fruit pericarp is described in light of the high-quality genome sequence. The proteomes of fruit pericarp from 12 tomato genotypes at two developmental stages (cell expansion and orange-red) were analyzed. The 2DE reference map included 506 spots identified by nano-LC/MS and the International Tomato Annotation Group Database searching. A total of 425 spots corresponded to un  ...[more]

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