Dataset Information

Human exosome proteome - Human urinary exosomes as innate immune effectors

ABSTRACT: Proteomics study of human urine exosomes. This data describes the proteomic complement of the human exosomal urine fraction from 10 healthy volunteers (5 male, 5 female) aged 23 to 36 years. 50 µg protein from each sample were fragmented on a 4%/12% SDS-polyacrylamide gel. Following staining, each gel track was separated into 28 equal sections, which were processed individually. Peptides were separated by reverse-phase chromatography (Dionex, Sunnyvale, CA) and LC-MS/MS was performed using an Eksigent NanoLC-1D Plus (Eksigent Technologies, Dublin, CA) HPLC system and an LTQ Orbitrap mass spectrometer (ThermoFisher, Waltham, MA). Peptides from each gel segment were analysed twice: (1) with a dynamic exclusion list and (2) a fixed exclusion list for the abundant protein uromodulin which was superimposed on a dynamic exclusion. MS data were processed using SEQUEST Bioworks Browser (version 3.3.1 SP1, ThermoFisher) to generate MS/MS peak lists. Combined peak list files were submitted to the MASCOT search algorithm (version 2.2.1, Matrix Science, London UK) and searched against the IPI-Human database.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine

SUBMITTER: Laurent Gatto

LAB HEAD: Kathryn Lilley

PROVIDER: PXD000117 | Pride | 2016-08-25

REPOSITORIES: Pride

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Human urinary exosomes as innate immune effectors.

Hiemstra Thomas F TF Charles Philip D PD Gracia Tannia T Hester Svenja S SS Gatto Laurent L Al-Lamki Rafia R Floto R Andres RA Su Ya Y Skepper Jeremy N JN Lilley Kathryn S KS Karet Frankl Fiona E FE

Journal of the American Society of Nephrology : JASN 20140403 9

Exosomes are small extracellular vesicles, approximately 50 nm in diameter, derived from the endocytic pathway and released by a variety of cell types. Recent data indicate a spectrum of exosomal functions, including RNA transfer, antigen presentation, modulation of apoptosis, and shedding of obsolete protein. Exosomes derived from all nephron segments are also present in human urine, where their function is unknown. Although one report suggested in vitro uptake of exosomes by renal cortical col ...[more]

PMID: 24700864

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			Action	DRS
	0909wa11svo2_21.RAW	Raw
	0909wa11svo2_21exl.RAW	Raw
	0909wa1svo1_1.RAW	Raw
	0909wa1svo1_1exl.RAW	Raw
	0909wa3svo1_9.RAW	Raw