Ontology highlight
ABSTRACT:
INSTRUMENT(S): TripleTOF 5600
ORGANISM(S): Rattus Norvegicus (rat)
SUBMITTER: Lekha Sleno
PROVIDER: PXD000128 | Pride | 2013-02-19
REPOSITORIES: Pride
Action | DRS | |||
---|---|---|---|---|
PTa.group | Other | |||
PTa.group.xml | Xml | |||
PTa_1_1.wiff | Wiff | |||
PTa_1_1.wiff.mtd | Wiff | |||
PTa_1_1.wiff.scan | Wiff |
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Journal of proteomics 20130220
A systematic approach was developed to optimize the analysis of rat liver microsomes combining ion exchange fractionation with reverse-phase chromatography coupled to high resolution quadrupole-time-of-flight mass spectrometry. A comparison was performed with several conditions to select the most efficient solubilization and proteolysis protocol to achieve highest proteome coverage. Optimal trypsin digestion conditions were achieved with SDS and heat to increase solubilization of microsomal samp ...[more]