Proteomics

Dataset Information

0

AKT2 interacting proteins_ MBP- and MAP-TAPs

ABSTRACT: Tagged AKT2 was expressed in HEK293T cells. For quantification SILAC labeling was performed. MBP-TAP: Cell lysates were mixed at a 1:1 ratio with unlabeled wild type cells before Tandem Affinity Purification. MAP-TAP: Same amount of labeled and unlabeled cell lysates were purified via Tandem Affinity Purification and afterwards eluates were mixed at a 1:1 ratio. AKT2 and co-purified proteins were digested with trypsin, fractionated via SCX and analyzed via LC-MS. Sequence database-search of the MS data was performed against the uniprot human taxonomy-9606 database containing 83659 entries using the SEQUEST algorithm with the following parameters: trypsin specificity, two missed cleavage sites, precurser ion mass accuracy tolerance of 10–30 ppm, cysteine carbamidomethylation, methionine oxidation, pSTY, N-terminal protein acetylation and, when performed, SILAC labels Lys-6, Arg-6 specified as modifications. The minimal cross-correlation score (XCorr) was set to 2.0, 2.5 and 3.0 for charge states +2, +3 and +4 respectively. The Delta Cn had to be >0.1 and the minimal peptide probability allowed was 0.05. The minimum number of peptides necessary for protein identification was three.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293 Cell, Hek-293t Cell

SUBMITTER: Katharina Bottermann  

LAB HEAD: Katharina Bottermann

PROVIDER: PXD000197 | Pride | 2013-08-13

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
110706_10.RAW Raw
110706_18.RAW Raw
110706_19.RAW Raw
110706_2.RAW Raw
110706_20.RAW Raw
Items per page:
1 - 5 of 756
altmetric image

Publications

Systematic Analysis Reveals Elongation Factor 2 and α-Enolase as Novel Interaction Partners of AKT2.

Bottermann Katharina K   Reinartz Michael M   Barsoum Marian M   Kötter Sebastian S   Gödecke Axel A  

PloS one 20130618 6

AKT2 is one of the three isoforms of the protein kinase AKT being involved in the modulation of cellular metabolism. Since protein-protein interactions are one possibility to convey specificity in signal transduction, we performed AKT2-protein interaction analysis to elucidate their relevance for AKT2-dependent cellular functions. We identified heat shock protein 90 kDa (HSP90), Cdc37, heat shock protein 70 kDa (HSP70), 78 kDa glucose regulated protein (GRP78), tubulin, GAPDH, α-enolase and elon  ...[more]

Similar Datasets

Transcriptomics Expansion of hepatic tumor progenito cell population in PTEN deficient mice requires liver injury and is reversed by deletion of Akt2
2016-03-31 | E-GEOD-70501 | biostudies-arrayexpress
Other Purification of TAP::ALG-1 complexes and associated RNA from mixed-stage TAP::ALG-1 transgenic (WS4303) and wild-type (N2) animals
2012-03-01 | GSE32941 | GEO
Genomics
| PRJNA669077 | ENA
Transcriptomics Identification of miRNA target genes in C. elegans by RIP-chip-SRM
2012-03-01 | E-GEOD-32944 | biostudies-arrayexpress
Transcriptomics Expansion of hepatic tumor progenito cell population in PTEN deficient mice requires liver injury and is reversed by deletion of Akt2
2016-03-31 | GSE70501 | GEO
Proteomics Analysis of mixed-oxysterol treatment on the THP-1 macrophage
2017-03-15 | PXD004304 | Pride
Transcriptomics Wnt Signaling Mediates Oncogenic Synergy Between Akt and Dlx5 in T-Cell Lymphomagenesis by Enhancing Cholesterol Synthesis [WTvsDP mouse RNA-seq]
2020-10-07 | GSE149349 | GEO
Transcriptomics Wnt Signaling Mediates Oncogenic Synergy Between Akt and Dlx5 in T-Cell Lymphomagenesis by Enhancing Cholesterol Synthesis [mouse PKF RNA-seq]
2020-10-07 | GSE149348 | GEO
Genomics Wnt Signaling Mediates Oncogenic Synergy Between Akt and Dlx5 in T-Cell Lymphomagenesis by Enhancing Cholesterol Synthesis [TCF ChIP]
2020-10-07 | GSE149347 | GEO
Transcriptomics Wnt Signaling Mediates Oncogenic Synergy Between Akt and Dlx5 in T-Cell Lymphomagenesis by Enhancing Cholesterol Synthesis [primary tumor RNA-seq]
2020-10-07 | GSE149346 | GEO