Dataset Information

Archael ubiquitin-like SAMP3 is isopeptide-linked to Proteins by an UbaA-dependent mechanism

ABSTRACT: FLAG-SAMP3ylated proteins were immunoprecipitated from Haloferax volcanii using anti-FLAG affinity agarose and separated using SDS-PAGE. The Coomassie-stained IP-bands represented FLAG-SAMP3ylated proteins harvested from the wild type and also from the ubaA mutant as negative control. The Coomassie-lanes from the IP samples were cut into 10 fractions and in-gel tryptic digested and the tryptic peptides measured using Orbitrap Velos LC-MS/MS.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Haloferax Volcanii (halobacterium Volcanii)

SUBMITTER: Haike Antelmann

LAB HEAD: Haike Antelmann

PROVIDER: PXD000202 | Pride | 2020-07-02

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	110224_OV3_P3_BH_HA_SACO_1_1.raw	Raw
	110224_OV3_P3_BH_HA_SACO_1_10.raw	Raw
	110224_OV3_P3_BH_HA_SACO_1_2.raw	Raw
	110224_OV3_P3_BH_HA_SACO_1_3.raw	Raw
	110224_OV3_P3_BH_HA_SACO_1_4.raw	Raw

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Publications

The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics.

Schulze Stefan S Adams Zachary Z Cerletti Micaela M De Castro Rosana R Ferreira-Cerca Sébastien S Fufezan Christian C Giménez María Inés MI Hippler Michael M Jevtic Zivojin Z Knüppel Robert R Legerme Georgio G Lenz Christof C Marchfelder Anita A Maupin-Furlow Julie J Paggi Roberto A RA Pfeiffer Friedhelm F Poetsch Ansgar A Urlaub Henning H Pohlschroder Mechthild M

Nature communications 20200619 1

While many aspects of archaeal cell biology remain relatively unexplored, systems biology approaches like mass spectrometry (MS) based proteomics offer an opportunity for rapid advances. Unfortunately, the enormous amount of MS data generated often remains incompletely analyzed due to a lack of sophisticated bioinformatic tools and field-specific biological expertise for data interpretation. Here we present the initiation of the Archaeal Proteome Project (ArcPP), a community-based effort to comp ...[more]

PMID: 32561711

Publication: 1/2

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