Dataset Information

Host-centric proteomics of stool

ABSTRACT: Soluble extra-cellular proteins and peptides from human and mouse stool were fractionated on a C-4 solid phase extraction cartidge, trypsin digested, C-18 clean up and identified with MS/MS on a LTQ-Velos Orbitrap. Data processing: The readRAW program was used to generate peak lists from the original data. All protein sequences were reversed and concatenated to the original forward-oriented proteins. Spectra were assigned to peptides using a semi-specific enzyme specificity with the Mascot search algorithm (version 2.3.01) using static carbamidomethylation of cysteines, differential oxidized methionines, 50ppm precursor mass tolerance, 2 miscleavages and 0.8Da fragment ion tolerance. Searched against a mouse or human database.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

SUBMITTER: Joshua Lichtman

PROVIDER: PXD000240 | Pride | 2013-10-16

REPOSITORIES: Pride

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Publications

Host-centric proteomics of stool: a novel strategy focused on intestinal responses to the gut microbiota.

Lichtman Joshua S JS Marcobal Angela A Sonnenburg Justin L JL Elias Joshua E JE

Molecular & cellular proteomics : MCP 20130827 11

The diverse community of microbes that inhabits the human bowel is vitally important to human health. Host-expressed proteins are essential for maintaining this mutualistic relationship and serve as reporters on the status of host-microbiota interaction. Therefore, unbiased and sensitive methods focused on host proteome characterization are needed. Herein we describe a novel method for applying shotgun proteomics to the analysis of feces, focusing on the secreted host proteome. We have conducted ...[more]

PMID: 23982161

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