Proteomics

Dataset Information

0

Host response profile of human brain proteome in toxoplasma encephalitis


ABSTRACT: Frontal cortex brain tissues obtained from 5 toxoplasma encephalitis patients and 5 controls were used for protein extraction. Protein amount was normalized on 10% SDS-PAGE, equal amount of protein from 5 patients and 5 controls were pooled separately and subjected to reduction, alkylation and trypsin digestion. iTRAQ labelling of samples were carried out in replicates. Peptides from control samples were labelled with iTRAQ labels leading to the reporter ions of 114 and 115 whereas peptides from patient samples were labelled with iTRAQ labels leading to the reporter ions of 116 and 117. Labelled peptides were then pooled and subjected to SCX fractionation and obtained 21 fractions, which were subsequently subjected to high-resolution Fourier transform mass spectrometry (LTQ-Orbitrap Velos).

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

SUBMITTER: Akhilesh Pandey  

LAB HEAD: Akhilesh Pandey

PROVIDER: PXD000261 | Pride | 2016-05-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
ToxoEncep_iTRAQ_01.msf Msf
ToxoEncep_iTRAQ_01.pep.xml Pepxml
ToxoEncep_iTRAQ_01.raw Raw
ToxoEncep_iTRAQ_02-01.msf Msf
ToxoEncep_iTRAQ_02-01.pep.xml Pepxml
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Publications


<h4>Background</h4>Toxoplasma encephalitis is caused by the opportunistic protozoan parasite Toxoplasma gondii. Primary infection with T. gondii in immunocompetent individuals remains largely asymptomatic. In contrast, in immunocompromised individuals, reactivation of the parasite results in severe complications and mortality. Molecular changes at the protein level in the host central nervous system and proteins associated with pathogenesis of toxoplasma encephalitis are largely unexplored. We u  ...[more]

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