Dataset Information

Analysis of mouse hippocampus membrane proteome by gel-based LC/MS

ABSTRACT: Mouse hippocampus membrane fractions were prepared using sucrose density gradient ultracentrifugation. Membrane proteins were solubilized using three different condition; 6-ACA/Triton X-100 or ProteoExtract Native Membrane Protein Extraction Kit (Calbiochem, Cat. No. 444810) or ProteoExtract Transmembrane Protein Extraction Kit (Novagen, Cat. No. 71772-3). Solubilized membrane proteins were separated on Blue Native PAGE or SDS-PAGE. Protein bands were excised, destained for peptide sample preparation. Proteins were reduced (DTT), alkylated (iodoacetamide), and in-gel digested with chymotrypsin or trypsin. Digested peptides were extracted then subjected to nanoHPLC and tandem MS analysis with Thermo Orbitrap Velos Pro mass spectrometer.

REANALYSED by: PAe005209PAe005204

INSTRUMENT(S): LTQ Orbitrap Velos Pro

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Hippocampus

SUBMITTER: Gert Lubec

LAB HEAD: Gert Lubec

PROVIDER: PXD000311 | Pride | 2014-08-15

REPOSITORIES: Pride

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Publications

Gel-based mass spectrometric analysis of hippocampal transmembrane proteins using high resolution LTQ Orbitrap Velos Pro.

Heo Seok S Spoerk Stefan S Birner-Gruenberger Ruth R Lubec Gert G

Proteomics 20140813 17-18

Membrane proteins (MPs) play diverse important roles for physical interactions, cell communication, molecular transport, and signal transduction. Membrane proteins comprise approximately 25∼35% of the genome in living organisms, but there are difficulties in the analysis at the protein chemical level, in particular due to low abundance and limited solubility. Sequence information on membrane proteins and their complexes would be beneficial to elucidate their function. Proteins were extracted fro ...[more]

PMID: 25044505

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	F017548.dat-ACA-BN_T1.raw	Raw
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	F017549.dat-ACA-BN_C1.raw	Raw
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