Proteomics

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Salmonella secretome analysis - Discovery of novel secreted virulence factors from Salmonella enterica serovar Typhimurium by proteomic analysis of culture supernatants


ABSTRACT: Researchers identified effector proteins secreted into defined minimal medium designed to induce expression of the SPI-2 TTSS and its effectors. The secretome of the parent strain was compared to those of strains missing essential (ssaK::cat) or regulatory (ssaL) components of the SPI-2 TTSS. 20 known SPI-2 effectors were identified. To identify novel effector proteins, the secretome data was coupled with machine learning and 12 candidate proteins were selected for further characterization. Using CyaA reporter fusions, six novel type III effectors were identified and two additional proteins that were secreted into J774 macrophages independently of a TTSS were also identified.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Salmonella Typhimurium (strain 14028s / Sgsc 2262)

SUBMITTER: Matthew Monroe  

PROVIDER: PXD000391 | Pride | 2013-10-07

REPOSITORIES: Pride

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Publications

Discovery of novel secreted virulence factors from Salmonella enterica serovar Typhimurium by proteomic analysis of culture supernatants.

Niemann George S GS   Brown Roslyn N RN   Gustin Jean K JK   Stufkens Afke A   Shaikh-Kidwai Afshan S AS   Li Jie J   McDermott Jason E JE   Brewer Heather M HM   Schepmoes Athena A   Smith Richard D RD   Adkins Joshua N JN   Heffron Fred F  

Infection and immunity 20101025 1


Salmonella enterica serovar Typhimurium is a leading cause of acute gastroenteritis throughout the world. This pathogen has two type III secretion systems (TTSS) encoded in Salmonella pathogenicity islands 1 and 2 (SPI-1 and SPI-2) that deliver virulence factors (effectors) to the host cell cytoplasm and are required for virulence. While many effectors have been identified and at least partially characterized, the full repertoire of effectors has not been catalogued. In this proteomic study, we  ...[more]

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