Dataset Information

Chromosome 11-Centric Human Proteome Project

ABSTRACT: For the analysis of chromosome 11-centric human proteome, we analyzed the human brain hippocampal proteome. The samples from brain tissues were extensively off-gel fractionated by 12 fractions and analyzed by a high resolution mass spectrometer, Orbitrap XL ETD with collision induced dissociation, after LC separation using C18 column.

INSTRUMENT(S): LTQ Orbitrap XL ETD

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

SUBMITTER: Kyung-Hoon Kwon

LAB HEAD: Jinyoung Kim

PROVIDER: PXD000395 | Pride | 2016-10-10

REPOSITORIES: Pride

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Publications

Chromosome-Based Proteomic Study for Identifying Novel Protein Variants from Human Hippocampal Tissue Using Customized neXtProt and GENCODE Databases.

Hwang Heeyoun H Park Gun Wook GW Kim Kwang Hoe KH Lee Ju Yeon JY Lee Hyun Kyoung HK Ji Eun Sun ES Park Sung-Kyu Robin SK Xu Tao T Yates John R JR Kwon Kyung-Hoon KH Park Young Mok YM Lee Hyoung-Joo HJ Paik Young-Ki YK Kim Jin Young JY Yoo Jong Shin JS

Journal of proteome research 20151116 12

The goal of the Chromosome-Centric Human Proteome Project (C-HPP) is to fully provide proteomic information from each human chromosome, including novel proteoforms, such as novel protein-coding variants expressed from noncoding genomic regions, alternative splicing variants (ASVs), and single amino acid variants (SAAVs). In the 144 LC/MS/MS raw files from human hippocampal tissues of control, epilepsy, and Alzheimer's disease, we identified the novel proteoforms with a workflow including integra ...[more]

PMID: 26549206

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