Proteomics

Dataset Information

0

Erythrocyte Proteome & Terminome


ABSTRACT: Proteins and protein N termini were isolated and enriched from human erythrocytes following the TAILS procedure with heavy dimethyl labeling of free N-termini (Kleifeld et al. Nat Biothech 2010). Following LC-MS/MS analysis on a Thermo Orbitrap XL spectra were matched using Mascot, X!Tandem and MSGF+ databases search engines and trans proteomic pipeline (TPP) modules for statistical evaluation. Maximum peptide level FDR was set to 1% (iProphet probability 0.88) and protein level FDR to 0.7% (proteinProphet probability 0.9).

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Philipp Lange  

PROVIDER: PXD000434 | Pride | 2014-02-24

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
111205_RBC5_ST_Fr10_11.RAW Raw
111205_RBC5_ST_Fr12.RAW Raw
111205_RBC5_ST_Fr13.RAW Raw
111205_RBC5_ST_Fr14.RAW Raw
111205_RBC5_ST_Fr15.RAW Raw
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Publications

Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome.

Lange Philipp F PF   Huesgen Pitter F PF   Nguyen Karen K   Overall Christopher M CM  

Journal of proteome research 20140310 4


A goal of the Chromosome-centric Human Proteome Project is to identify all human protein species. With 3844 proteins annotated as "missing", this is challenging. Moreover, proteolytic processing generates new protein species with characteristic neo-N termini that are frequently accompanied by altered half-lives, function, interactions, and location. Enucleated and largely void of internal membranes and organelles, erythrocytes are simple yet proteomically challenging cells due to the high hemogl  ...[more]

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