Proteomics

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Chr16-HPP. Shotgun Analysis improvement. JPR HPP Special issue 2013. Ramos Cell line.

ABSTRACT: The Chromosome 16 Consortium is integrated in the Human Proteome Project that aims to develop an entire map of the proteins encoded by the human genome following a gene-centric strategy (C-HPP) to make progress in the understanding of human biology in health and disease (B/D-HPP). To do this study four human cell lines were selected, MCF7 epithelial cells, CCD18 colon fibroblasts, Ramos and Jurkat B and T lymphocytes respectively. In particular, this subset includes all the data regarding the Ramos cell line. 3 laboratories contributed to compile all the reported data.

REANALYSED by: PAe005043PAe005023PAe004992

INSTRUMENT(S): LTQ Orbitrap Velos, Thermo Fisher Scientific instrument model

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Ramos Cell

SUBMITTER: J. Alberto Medina-Aunon  

LAB HEAD: J. Alberto Medina-Aunon

PROVIDER: PXD000447 | Pride | 2013-09-05

REPOSITORIES: Pride

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Publications

Surfing transcriptomic landscapes. A step beyond the annotation of chromosome 16 proteome.

Segura Víctor V   Medina-Aunon Juan Alberto JA   Mora Maria I MI   Martínez-Bartolomé Salvador S   Abian Joaquín J   Aloria Kerman K   Antúnez Oreto O   Arizmendi Jesús M JM   Azkargorta Mikel M   Barceló-Batllori Silvia S   Beaskoetxea Jabier J   Bech-Serra Joan J JJ   Blanco Francisco F   Monteiro Mariana B MB   Cáceres David D   Canals Francesc F   Carrascal Monserrat M   Casal José Ignacio JI   Clemente Felipe F   Colomé Nuria N   Dasilva Noelia N   Díaz Paula P   Elortza Félix F   Fernández-Puente Patricia P   Fuentes Manuel M   Gallardo Oscar O   Gharbi Severine I SI   Gil Concha C   González-Tejedo Carmen C   Hernáez María Luisa ML   Lombardía Manuel M   Lopez-Lucendo Maria M   Marcilla Miguel M   Mato José M JM   Mendes Marta M   Oliveira Eliandre E   Orera Irene I   Pascual-Montano Alberto A   Prieto Gorka G   Ruiz-Romero Cristina C   Sánchez del Pino Manuel M MM   Tabas-Madrid Daniel D   Valero Maria L ML   Vialas Vital V   Villanueva Joan J   Albar Juan Pablo JP   Corrales Fernando J FJ  

Journal of proteome research 20131105 1

The Spanish team of the Human Proteome Project (SpHPP) marked the annotation of Chr16 and data analysis as one of its priorities. Precise annotation of Chromosome 16 proteins according to C-HPP criteria is presented. Moreover, Human Body Map 2.0 RNA-Seq and Encyclopedia of DNA Elements (ENCODE) data sets were used to obtain further information relative to cell/tissue specific chromosome 16 coding gene expression patterns and to infer the presence of missing proteins. Twenty-four shotgun 2D-LC-MS  ...[more]

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