Dataset Information

Validation dataset on tamoxifen treated Estrogen Receptor (ER) positive breast cancer patients

ABSTRACT: 56 Estrogen Receptor Positive Breast cancer tissues coming from patients treated with tamoxifen upon recurrence (41 Good outcome vs 15 Poor outcome) have been microdissected to enrich for breast cancer tumor cells. Enriched tissue has been lysed and proteins digested and analyzed in an LTQ-Orbitrap coupled online with a Dionex nLC system. Thermo output .RAW files were analyzed through MaxQuant (version 1.4.1.2) using the human proteome FASTA (version 2012-09, human canonical proteome) as search library. LFQ parameters in MaxQuant have been enabled (Fast LFQ) and other settings have been kept as default.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Tommaso De Marchi

LAB HEAD: Tommaso De Marchi

PROVIDER: PXD000485 | Pride | 2015-08-21

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	121004OTc2_TDM-AU-0324-N-1_008.RAW	Raw
	121004OTc2_TDM-AU-0324-N-2_017.RAW	Raw
	121004OTc2_TDM-AU-0324-N-3_028.RAW	Raw
	121004OTc2_TDM-AU-0324-NKI-1_014.RAW	Raw
	121004OTc2_TDM-AU-0324-NKI-2_025.RAW	Raw

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Publications

4-protein signature predicting tamoxifen treatment outcome in recurrent breast cancer.

De Marchi Tommaso T Liu Ning Qing NQ Stingl Cristoph C Timmermans Mieke A MA Smid Marcel M Look Maxime P MP Tjoa Mila M Braakman Rene B H RB Opdam Mark M Linn Sabine C SC Sweep Fred C G J FC Span Paul N PN Kliffen Mike M Luider Theo M TM Foekens John A JA Martens John W M JW Umar Arzu A

Molecular oncology 20150807 1

Estrogen receptor (ER) positive tumors represent the majority of breast malignancies, and are effectively treated with hormonal therapies, such as tamoxifen. However, in the recurrent disease resistance to tamoxifen therapy is common and a major cause of death. In recent years, in-depth proteome analyses have enabled identification of clinically useful biomarkers, particularly, when heterogeneity in complex tumor tissue was reduced using laser capture microdissection (LCM). In the current study, ...[more]

PMID: 26285647

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