Proteomics

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2D-LC/MS/MS analysis of Escherichia coli during exposure an abrupt downshift in water activity


ABSTRACT: 2D-LC/MS/MS analysis was used to examine time-dependent changes in proteome of E. coli O157:H7 strain Sakai upon an abrupt downshift in water activity (aw) (i.e., from aw 0.993 to aw 0.967) at a constant temperature of 35°C. Bacterial cells were harvested before hyperosmotic shift, and 0 (i.e. immediately after the shift), 30, 80, and 310 min after the shift. It also should be noted that these time points were chosen with the aims to characterize the physiology of E. coli during dynamic changes in growth kinetics induced by an abrupt downshift in water activity. Specifically, the samples taken at time 0 and 30 min were obtained during the period in which the loss of cell culturability was observed, whereas the samples at time 80 and 310 min respectively reflected the physiological state of E. coli during the ‘recovery’ period and during growth after the shift.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Escherichia Coli

SUBMITTER: Chawalit Kocharunchitt  

PROVIDER: PXD000541 | Pride | 2014-04-14

REPOSITORIES: Pride

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Publications

Global genome response of Escherichia coli O157∶H7 Sakai during dynamic changes in growth kinetics induced by an abrupt downshift in water activity.

Kocharunchitt Chawalit C   King Thea T   Gobius Kari K   Bowman John P JP   Ross Tom T  

PloS one 20140303 3


The present study was undertaken to investigate growth kinetics and time-dependent change in global expression of Escherichia coli O157∶H7 Sakai upon an abrupt downshift in water activity (aw). Based on viable count data, shifting E. coli from aw 0.993 to aw 0.985 or less caused an apparent loss, then recovery, of culturability. Exponential growth then resumed at a rate characteristic for the aw imposed. To understand the responses of this pathogen to abrupt osmotic stress, we employed an integr  ...[more]

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