Dataset Information

Large-scale proteome characterization of the cassava root

ABSTRACT: Cassava is the most important root crop in the tropics but rapid post-harvest physiological root deterioration (PPD) is a major constraint to commercial cassava production. We used label-free quantitative proteomics to generate an extensive cassava root and PPD proteome. Over 2400 unique proteins were identified in the cassava root and nearly 300 proteins showed significant abundance regulation during PPD. A candidate gene for reducing PPD was identified from the regulated proteins with enzymatic assays and afterwards verified with a transgene approach. This demonstrates the relevance of proteomics approach for crop improvements.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Manihot Esculenta (cassava) (jatropha Manihot)

TISSUE(S): Root

SUBMITTER: Katja Baerenfaller

LAB HEAD: Herve Vanderschuren

PROVIDER: PXD000587 | Pride | 2014-06-03

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20110303_07_root1_0h_F5.RAW	Raw
	20110303_07_root1_0h_F5.mgf	Mgf
	20110303_08_root1_6h_F5.RAW	Raw
	20110303_08_root1_6h_F5.mgf	Mgf
	20110303_09_root1_12h_F5.RAW	Raw

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Publications

Large-Scale Proteomics of the Cassava Storage Root and Identification of a Target Gene to Reduce Postharvest Deterioration.

Vanderschuren Hervé H Nyaboga Evans E Poon Jacquelyne S JS Baerenfaller Katja K Grossmann Jonas J Hirsch-Hoffmann Matthias M Kirchgessner Norbert N Nanni Paolo P Gruissem Wilhelm W

The Plant cell 20140529 5

Cassava (Manihot esculenta) is the most important root crop in the tropics, but rapid postharvest physiological deterioration (PPD) of the root is a major constraint to commercial cassava production. We established a reliable method for image-based PPD symptom quantification and used label-free quantitative proteomics to generate an extensive cassava root and PPD proteome. Over 2600 unique proteins were identified in the cassava root, and nearly 300 proteins showed significant abundance regulati ...[more]

PMID: 24876255

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