Proteomics

Dataset Information

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The human plasma proteome before and after remote ischemic preconditioning

ABSTRACT: Six healthy male adults underwent RIPC, consisting of 3x5 min cycles of upper arm ischemia (inflating a blood pressure cuff to >200 mmHg) alternating with 3x5 min reperfusion (bp cuff deflated). Blood samples were collected at baseline and at 1 and 4 min into each reperfusion period. Plasma was isolated and the six reperfusion samples were pooled to one RIPC sample. Both baseline and RIPC samples were depleted, concentrated, trypsin digested, iTRAQ labeled and analyzed by LC and Orbitrap-MS where each RIPC sample was compared to the same individual's baseline sample.

REANALYSED by: PAe005195

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Blood Plasma

SUBMITTER: Marc Vaudel  

LAB HEAD: Marc Vaudel

PROVIDER: PXD000605 | Pride | 2014-09-16

REPOSITORIES: Pride

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EH_Exp1_1.raw Raw
EH_Exp1_10.raw Raw
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Publications

Exploring the human plasma proteome for humoral mediators of remote ischemic preconditioning--a word of caution.

Helgeland Erik E   Breivik Lars Ertesvåg LE   Vaudel Marc M   Svendsen Øyvind Sverre ØS   Garberg Hilde H   Nordrehaug Jan Erik JE   Berven Frode Steingrimsen FS   Jonassen Anne Kristine AK  

PloS one 20141015 10

Despite major advances in early revascularization techniques, cardiovascular diseases are still the leading cause of death worldwide, and myocardial infarctions contribute heavily to this. Over the past decades, it has become apparent that reperfusion of blood to a previously ischemic area of the heart causes damage in and of itself, and that this ischemia reperfusion induced injury can be reduced by up to 50% by mechanical manipulation of the blood flow to the heart. The recent discovery of rem  ...[more]