Dataset Information

Genomics and Proteomics Provide New Insight into the Commensal and Pathogenic Lifestyles of Bovine and Human-associated Staphylococcus epidermidis

ABSTRACT: The goal of the present study is to understand mechanisms by which SE can infect the bovine host and persist in the mammary gland. For this purpose we determined the whole genome and four plasmid sequences of the SE strain PM221 originating from spontaneous persistent bovine IMI. We compared the genomic data with those of ATCC12228 and RP62A to screen for conserved and acquired gene pools. To confirm and complement these findings two-dimensional difference gel electrophoresis (2D DIGE) involving each three strains, surfome shaving and experimental infections in a bovine model was applied to indicate potential differences.

OTHER RELATED OMICS DATASETS IN: PRJEB5186

INSTRUMENT(S): QSTAR

ORGANISM(S): Staphylococcus Epidermidis

SUBMITTER: Pia SiljamÃ¤ki

LAB HEAD: Tuula A Nyman

PROVIDER: PXD000683 | Pride | 2014-07-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	131203PM221PIIkelt.wiff	Wiff
	131203PM221PIIkelt.wiff.scan	Wiff
	131203PM221PIIvihr.wiff	Wiff
	131203PM221PIIvihr.wiff.scan	Wiff
	131203PM221PIjaPIIkeltFDRMascotF007162.dat	Other

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Publications

Genomics and Proteomics Provide New Insight into the Commensal and Pathogenic Lifestyles of Bovine- and Human-Associated Staphylococcus epidermidis Strains.

Savijoki Kirsi K Iivanainen Antti A Siljamäki Pia P Laine Pia K PK Paulin Lars L Karonen Taru T Pyörälä Satu S Kankainen Matti M Nyman Tuula A TA Salomäki Tiina T Koskinen Patrik P Holm Liisa L Simojoki Heli H Taponen Suvi S Sukura Antti A Kalkkinen Nisse N Auvinen Petri P Varmanen Pekka P

Journal of proteome research 20140718 8

The present study reports comparative genomics and proteomics of Staphylococcus epidermidis (SE) strains isolated from bovine intramammary infection (PM221) and human hosts (ATCC12228 and RP62A). Genome-level profiling and protein expression analyses revealed that the bovine strain and the mildly infectious ATCC12228 strain are highly similar. Their genomes share high sequence identity and synteny, and both were predicted to encode the commensal-associated fdr marker gene. In contrast, PM221 was ...[more]

PMID: 25014494

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