Dataset Information

Altered microbiota-host mutualism and bloom of Atopobium define severe pediatric Crohn's disease

ABSTRACT: Microbial dysbiosis has been identified in adult inflammatory bowel disease (IBD) patients. However, microbial composition and functional interplay between host genetics and microorganisms in early IBD onset remain poorly defined. Here, we identified and demonstrated the causal effect of Atopobium parvulum and the gut microbiota in pediatric IBD. Microbiota and proteomic profiling revealed that the abundance of A. parvulum, a potent H2S producer, was associated with increased disease severity and a concurrent reduction in the expression of the host H2S detoxification pathway. In the Il10-/- mouse model of inflammation, A. parvulum induced severe pancolitis that was dependent on the presence of the gut microbiota. In addition, we demonstrated that administration of bismuth, an H2S scavenger, prevented A. parvulum-induced colitis. Our findings identified Atopobium parvulum as a major mediator of inflammation severity, and revealed an alteration of the balance between the production and detoxification of H2S in the gastrointestinal tract.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colon

SUBMITTER: Amanda Starr

LAB HEAD: Daniel Figeys

PROVIDER: PXD000690 | Pride | 2022-02-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MaxQuantOutput.tar.gz	Other
	Shelley_20130729_CD_CoA271_Severe_F1.raw	Raw
	Shelley_20130729_CD_CoA271_Severe_F2.raw	Raw
	Shelley_20130729_CD_CoA271_Severe_F3.raw	Raw
	Shelley_20130729_CD_CoA271_Severe_F4.raw	Raw

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Publications

Altered intestinal microbiota-host mitochondria crosstalk in new onset Crohn's disease.

Mottawea Walid W Chiang Cheng-Kang CK Mühlbauer Marcus M Starr Amanda E AE Butcher James J Abujamel Turki T Deeke Shelley A SA Brandel Annette A Zhou Hu H Shokralla Shadi S Hajibabaei Mehrdad M Singleton Ruth R Benchimol Eric I EI Jobin Christian C Mack David R DR Figeys Daniel D Stintzi Alain A

Nature communications 20161123

Intestinal microbial dysbiosis is associated with Crohn's disease (CD). However, the mechanisms leading to the chronic mucosal inflammation that characterizes this disease remain unclear. In this report, we use systems-level approaches to study the interactions between the gut microbiota and host in new-onset paediatric patients to evaluate causality and mechanisms of disease. We report an altered host proteome in CD patients indicative of impaired mitochondrial functions. In particular, mitocho ...[more]

PMID: 27876802

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