Dataset Information

Protein expression in beta-carotene transgenic soybeans

ABSTRACT: Soybeans (Glycine max) were genetically modified using a gene gun to produce beta-carotene, which is not normally found in the species. beta-carotene is produced in the chloroplast in which it competes with chlorophyl for production. A proteomics analysis was performed to determine the effects of the transgene on seed protein content.

INSTRUMENT(S): LTQ Velos

ORGANISM(S): Glycine Max

TISSUE(S): Plant Cell, Seed

SUBMITTER: Ken Pendarvis

LAB HEAD: Monica Schmitd

PROVIDER: PXD000709 | Pride | 2016-07-19

REPOSITORIES: Pride

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Publications

Transgenic soya bean seeds accumulating β-carotene exhibit the collateral enhancements of oleate and protein content traits.

Schmidt Monica A MA Parrott Wayne A WA Hildebrand David F DF Berg R Howard RH Cooksey Amanda A Pendarvis Ken K He Yonghua Y McCarthy Fiona F Herman Eliot M EM

Plant biotechnology journal 20141114 4

Transgenic soya bean (Glycine max) plants overexpressing a seed-specific bacterial phytoene synthase gene from Pantoea ananatis modified to target to plastids accumulated 845 μg β carotene g(-1) dry seed weight with a desirable 12:1 ratio of β to α. The β carotene accumulating seeds exhibited a shift in oil composition increasing oleic acid with a concomitant decrease in linoleic acid and an increase in seed protein content by at least 4% (w/w). Elevated β-carotene accumulating soya bean cotyled ...[more]

PMID: 25400247

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			Action	DRS
	G1_D.mgf	Mgf
	G1_D.raw	Raw
	G1_S.mgf	Mgf
	G1_S.raw	Raw
	G1_Tr.mgf	Mgf