Dataset Information

Label-free mass spectrometry-based profiling of urinary proteins and metabolites for non-invasive diagnosis of idiopathic nephrotic syndrome in children

ABSTRACT: To identify specific urinary proteomics and metabolomics patterns associated with paediatric idiopathic nephrotic syndrome (INS).

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine, Kidney

DISEASE(S): Nephrotic Syndrome

SUBMITTER: Hans Vissers

LAB HEAD: Sandra Kraljevic Pavelic

PROVIDER: PXD000765 | Pride | 2014-08-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	INS_01a_001.mzML	Mzml
	INS_01a_001_PRIDE.pride.mgf.gz	Mgf
	INS_01a_001_PRIDE.pride.mztab.gz	Mztab
	INS_01a_001_PRIDE.xml.gz	Xml
	INS_01b_002.mzML	Mzml

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Publications

Label-free mass spectrometric profiling of urinary proteins and metabolites from paediatric idiopathic nephrotic syndrome.

Sedic Mirela M Gethings Lee A LA Vissers Johannes P C JP Shockcor John P JP McDonald Stephen S Vasieva Olga O Lemac Maja M Langridge James I JI Batinić Danica D Pavelić Sandra Kraljević SK

Biochemical and biophysical research communications 20140820 1

Idiopathic nephrotic syndrome (INS) is caused by renal diseases that increase the permeability of the glomerular filtration barrier without evidence of a specific systemic cause. The aim of the present work was to reveal inherent molecular features of INS in children using combined urinary proteomics and metabolomics profiling. In this study, label-free mass spectrometric analysis of urinary proteins and small molecule metabolites was carried out in 12 patients with INS versus 12 sex- and age-ma ...[more]

PMID: 25150443

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Project description:Background: Minimal change nephrotic syndrome (MCNS) is considered to be associated with T cell dysfunction, via unknown mechanisms. Experimental observations suggest that some humoral factors alter the permeability of glomerular filtration barrier. However, the nature of such factors remains still uncertain. Methods: Using cDNA microarrays, we performed gene expression profiling of peripheral blood mononuclear cells (PBMC) from three patients with MCNS during nephrosis and remission phases. To confirm the cDNA microarray results, we performed quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) analyses in nephrosis and remission samples from 20 MCNS patients and six patients with nephrotic syndrome due to membranous nephropathy. Results: Out of 24,446 genes screened, 33 genes were up-regulated (at least 1.5-fold) in PBMC from these MCNS patients during the nephrosis phase. Up-regulated genes mainly encoded proteins involved in signal transduction and cytokine response. For further examination, we selected two genes encoding provable secretary proteins, chemokine (C-C) ligand 13 (also known as monocyte chemotactic protein-4) (CCL13) and a novel galectin-related protein (HSPC159). The results of RT-PCR showed that expressions of CCL13 and HSPC159 mRNA in nephrosis PBMC samples are higher than those in remission PBMC samples from all 20 MCNS patients examined. On the other hand, these mRNA expression patterns were variable among six patients with membranous nephropathy. Conclusions: We conclude that CCL13 and HSPC159 mRNA expressions in PBMC is up-regulated in MCNS patients during the nephrosis phase. These expression changes in PBMC might be involved in the pathophysiologic processes of MCNS. Using cDNA microarrays, we performed gene expression profiling of peripheral blood mononuclear cells (PBMC) from three patients with MCNS during nephrosis and remission phases. To confirm the cDNA microarray results, we performed quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) analyses in nephrosis and remission samples from 20 MCNS patients and six patients with nephrotic syndrome due to membranous nephropathy.