Dataset Information

Proteomic characterisation of the salt gland-enriched tissues of the mangrove tree species Avicennia officinalis

ABSTRACT: Plant salt glands are nature’s desalination devices that harbour potentially useful information pertaining to salt and water transport during secretion. As part of the program toward deciphering secretion mechanisms in salt glands, we adopted a shotgun approach to look into the proteome of salt-gland enriched tissues of the mangrove tree species Avicennia officinalis. To achieve this, we isolated the adaxial epidermal peels (which harbour the salt glands), and separated them from the mesophyll tissues of the leaves. Three biological replicates were prepared and total proteins were extracted from these tissues. Proteins that were found to be unique in salt gland-enriched tissues were obtained by eliminating proteins found in the mesophyll tissues. The proteins that are uniquely present in salt gland-enriched tissues were then selected and categorized by GO analysis.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Avicennia

TISSUE(S): Leaf

SUBMITTER: Qingsong Lin

LAB HEAD: Qingsong Lin

PROVIDER: PXD000771 | Pride | 2015-07-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	131108_WK_T1_2DLC_F1_5ul.mzml	Mzml
	131108_WK_T1_2DLC_F2_5ul.mzml	Mzml
	131108_WK_T1_2DLC_F3_5ul.mzml	Mzml
	131108_WK_T1_2DLC_F4_5ul.mzml	Mzml
	131108_WK_T1_2DLC_F5_5ul.mzml	Mzml

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Publications

Proteomic Characterisation of the Salt Gland-Enriched Tissues of the Mangrove Tree Species Avicennia officinalis.

Tan Wee-Kee WK Lim Teck-Kwang TK Loh Chiang-Shiong CS Kumar Prakash P Lin Qingsong Q

PloS one 20150720 7

Plant salt glands are nature's desalination devices that harbour potentially useful information pertaining to salt and water transport during secretion. As part of the program toward deciphering secretion mechanisms in salt glands, we used shotgun proteomics to compare the protein profiles of salt gland-enriched (isolated epidermal peels) and salt gland-deprived (mesophyll) tissues of the mangrove species Avicennia officinalis. The purpose of the work is to identify proteins that are present in ...[more]

PMID: 26193361

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