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Mitral Valve Tissue Shotgun Proteomics

ABSTRACT: In this study, we carried out a large-scale proteome profiling of human mitral valve tissues resected from patients with mitral valve prolapse.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Heart

SUBMITTER: Hwee Tong Tan

LAB HEAD: Hwee Tong Tan

PROVIDER: PXD000774 | Pride | 2015-05-07

REPOSITORIES: Pride

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			Action	DRS
	130626_HT__Spl_129_shotgun_10ul.group.xml	Xml
	130626_HT__Spl_129_shotgun_10ul.mzml	Mzml
	130626_HT__Spl_6035_shotgun_10ul.group.xml	Xml
	130626_HT__Spl_6035_shotgun_10ul.mzml	Mzml
	130626_HT__Spl_72_shotgun_10ul.group.xml	Xml

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Publications

Unravelling the proteome of degenerative human mitral valves.

Tan Hwee Tong HT Lim Teck Kwang TK Richards Arthur Mark AM Kofidis Theodoros T Teoh Kristine Leok-Kheng KL Ling Lieng H LH Chung Maxey C M MC

Proteomics 20150608 17

Degenerative mitral valve disease (DMVD), which includes the syndromes of mitral valve prolapse (MVP) and flail leaflet, is a common valvular condition which can be complicated by mitral regurgitation and adverse cardiovascular outcomes. Although several genetic and other studies of MVP in dog models have provided some information regarding the underlying disease mechanisms, the proteins and molecular events mediating human MVP pathogenesis have not been unraveled. In this study, we report the f ...[more]

PMID: 25914152

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Mitral valve interstitial Tissue: Myxomatous P2 prolapse of the mitral valve vs. Control

Project description:Mitral valve interstitial Tissue: Myxomatous P2 prolapse of the mitral valve vs. Control

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Genomic expression patterns of mitral valve tissues from dogs with degenerative mitral valve disease

Project description:Evaluation of global expression patterns provides a molecular portrait of mitral valve disease, yields insight into the pathophysiologic aspects of DMVD, and identifies intriguing genes and pathways for further study. (Am J Vet Res 2006;67:1307–1318) Keywords: control vs diseased 4 controls( beagle crosses) and 4 affected (1 Daschund, 1 Lhasa apso, 2 miniature poodles)

2008-05-15 | E-GEOD-11460 | biostudies-arrayexpress

Genomic expression patterns of mitral valve tissues from dogs with degenerative mitral valve disease

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Normal human mitral valve proteome: a preliminary investigation by gel-based and gel-free proteomic approaches

Project description:The mitral valve is a highly complex structure which regulates blood flow from the left atrium to the left ventricle (LV) avoiding a significant forward gradient during diastole or regurgitation during systole. The integrity of the mitral valve is also essential for the maintenance of normal LV size, geometry, and function. Significant advances in the comprehension of the biological, functional, and mechanical behavior of the mitral valve have recently been made. However, current knowledge of protein components in the normal human mitral valve is still limited and complicated by the low cellularity of this tissue and the presence of high abundant proteins from the extracellular matrix. We employed here an integrated proteomic approach to analyse the protein composition of the normal human mitral valve and reported confident identification of 422 proteins, some of which have not been previously described in this tissue. In particular, we described the ability of pre-MS separation technique based on liquid-phase IEF and SDS-PAGE to identify the largest number of proteins. These initial results provide a valuable basis for future studies aimed at analysing in depth the mitral valve protein composition and at investigating potential pathogenetic molecular mechanisms.

2016-08-18 | PXD004397 | Pride

Understanding the cell fate and behavior of progenitors at the origin of the mouse cardiac mitral valve

Project description:Congenital heart malformations include mitral valve defects, which remain largely unexplained. During embryogenesis, a restricted population of endocardial cells within the atrioventricular canal undergoes an endothelial-to-mesenchymal transition to give rise to mitral valvular cells. However, the identity and fate decisions of these progenitors as well as the behavior and distribution of their derivatives in valve leaflets remain unknown. We used single-cell RNA sequencing (scRNA-seq) of genetically labeled endocardial cells and microdissected mouse embryonic and postnatal mitral valves to characterize the developmental road. We defined the metabolic processes underlying the specification of the progenitors and their contributions to subtypes of valvular cells. Using retrospective multicolor clonal analysis, we describe specific modes of growth and behavior of endocardial cell-derived clones, which build up, in a proper manner, functional valve leaflets. Our data identify how both genetic and metabolic mechanisms specifically drive the fate of a subset of endocardial cells toward their distinct clonal contribution to the formation of the valve.

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Project description:To characterize the cell types that compose the mitral valve during the development of autoimmune valvular carditis, we utilized scRNA sequencing. Live cells from inflamed valves of K/B.g7 mice or uninflamed B.g7 controls were collected and analyzed at 3-, 8-, and 25 weeks of age.

2024-01-01 | GSE221197 | GEO

Mitral valve transcriptome analysis in thirty-four age-matched Cavalier King Charles Spaniels with or without congestive heart failure caused by myxomatous mitral valve disease

Project description:We here report the results of a mitral valve transcriptome study designed to identify genes and molecular pathways involved in development of congestive heart failure (CHF) following myxomatous mitral valve disease (MMVD) in dogs. The study is focused on a cohort of elderly age-matched dogs (n=34, age ~10 years) from a single breed – Cavalier King Charles Spaniels (CKCS) – with a high incidence of MMVD. The cohort comprises 19 dogs (10♀, 9♂) without MMVD-associated CHF, and 15 dogs (6♀, 9♂) with CHF caused by MMVD. I.e. we compare gene expression in breed and age matched groups of dogs, which only differ with respect to CHF status. We identify 56 genes, which are differentially expressed between the two groups. In this list of genes, we confirm an enrichment of genes related to the TNFβ signaling pathway, extracellular matrix organization, vascular development, and endothelium damage, which also have been identified in previous studies. However, the genes with the greatest difference in expression between the two groups are CNTN3 and MYH1. Both genes encode proteins, which are predicted to have an effect on the contractile activity of myocardial cells, which in turn may have an effect on valvular performance and hemodynamics across the mitral valve. This may result in shear forces with impact on MMVD progression.

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Mitral valve leaflet response to ischemic mitral regurgitation: From gene expression to tissue remodeling

Project description:Mitral valve leaflet response to ischemic mitral regurgitation: From gene expression to tissue remodeling

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Genome-wide analysis of RNA binding proteins co-expression with alternative splicing events in mitral valve prolapse

Project description:For RNA extraction, we obtained peripheral blood mononuclear cells (PBMCs) from 5 patients with mitral valve prolapse (MVP), with or without chordae tendineae rupture, and 5 healthy individuals. High-throughput sequencing was used for RNA-sequencing.

2023-04-18 | GSE229778 | GEO

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