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H2A.Bbd localizes to DNA synthesis sites

ABSTRACT: Mass spectrometry analysis of chromatin-containing GFP-H2A.Bbd revealed an high enrichment of factors associated to replication compared to GFP-macroH2A.1.2 and GFP-H2A.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Andreas Schmidt

LAB HEAD: Axel Imhof

PROVIDER: PXD000789 | Pride | 2014-05-18

REPOSITORIES: Pride

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			Action	DRS
	MaxquantOutput.tar.gz	Other
	SID1166_120811_Bbd1.RAW	Raw
	SID1166_120811_Bbd2.RAW	Raw
	SID1166_120811_Bbd3.RAW	Raw
	SID1166_120811_Bbd4.RAW	Raw

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The histone variant H2A.Bbd is enriched at sites of DNA synthesis.

Sansoni Viola V Casas-Delucchi Corella S CS Rajan Malini M Schmidt Andreas A Bönisch Clemens C Thomae Andreas W AW Staege Martin S MS Hake Sandra B SB Cardoso M Cristina MC Imhof Axel A

Nucleic acids research 20140420 10

Histone variants play an important role in shaping the mammalian epigenome and their aberrant expression is frequently observed in several types of cancer. However, the mechanisms that mediate their function and the composition of the variant-containing chromatin are still largely unknown. A proteomic interrogation of chromatin containing the different H2A variants macroH2A.1.2, H2A.Bbd and H2A revealed a strikingly different protein composition. Gene ontology analysis reveals a strong enrichmen ...[more]

PMID: 24753410

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