Proteomics

Dataset Information

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Salivary proteins in buffalo with special emphasis to estrous cycle


ABSTRACT: Saliva is considered as the best source for biomarker-discovery studies, since it is a non-invasive method when compared to other body sources. Usually, buffalo cannot express their estrus signs precisely. Hence, there is a need for concise methods to detect the time of estrus to ensure the success in artificial insemination. Therefore, we have established a reference proteome map on buffalo whole saliva during estrous cycle in order to document the estrus-specific proteins using SDS-PAGE and mass spectrometry. The present findings conclude that the proteomic approach adopted to identify the proteins from buffalo saliva around estrous cycle may provide a new tool for screening the estrus phase.

INSTRUMENT(S): Thermo Electron instrument model

ORGANISM(S): Bubalus Bubalis

TISSUE(S): Saliva

SUBMITTER: Subramanian Muthukumar  

LAB HEAD: Govindaraju Archunan

PROVIDER: PXD000816 | Pride | 2016-07-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
E0316-Rama-R1.mgf Mgf
E0316-Rama-R1.mzXML Mzxml
E0316-Rama-R10.mgf Mgf
E0316-Rama-R10.mzXML Mzxml
E0316-Rama-R11.mgf Mgf
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Publications

Exploration of salivary proteins in buffalo: an approach to find marker proteins for estrus.

Muthukumar Subramanian S   Rajkumar Ramalingam R   Rajesh Durairaj D   Saibaba Ganesan G   Liao Chen-Chung CC   Archunan Govindaraju G   Padmanabhan Parasuraman P   Gulyas Balazs B  

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20140811 11


Saliva is considered as the best source of biological material for biomarker discovery studies since it is noninvasive in comparison to other body sources. Usually buffalo cannot precisely express estrus signals. Hence, there is a need for concise methods to detect the time of estrus to ensure the success of artificial insemination. Therefore, we have established a reference proteome map on the whole saliva of buffalo during their estrous cycle with special reference to estrus. Nearly 12 bands h  ...[more]

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