Dataset Information

Phosphoproteome profiles of the phytopathogenic fungi Alternaria brassicicola and Botrytis cinerea

ABSTRACT: This study describes the gel-free phosphoproteomic analysis the phytopathogenic fungi Alternaria brassicicola and Botrytis cinerea grown in vitro under non-limiting conditions.

INSTRUMENT(S): LTQ

ORGANISM(S): Alternaria Brassicae Botryotinia Fuckeliana (strain B05.10) (noble Rot Fungus) (botrytis Cinerea)

TISSUE(S): Mycelium

SUBMITTER: Marlène Davanture

LAB HEAD: Michel Zivy

PROVIDER: PXD000817 | Pride | 2014-06-09

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20110311_Fillinger_Alternaria_1711_01.mgf	Mgf
	20110311_Fillinger_Alternaria_1711_01.mzXML	Mzxml
	20110311_Fillinger_Alternaria_1711_01.xml-pride.pride.mgf.gz	Mgf
	20110311_Fillinger_Alternaria_1711_01.xml-pride.pride.mztab.gz	Mztab
	20110311_Fillinger_Alternaria_1711_01.xml-pride.xml.gz	Xml

Items per page:

1 - 5 of 640

Publications

Phosphoproteome profiles of the phytopathogenic fungi Alternaria brassicicola and Botrytis cinerea during exponential growth in axenic cultures.

Davanture Marlène M Dumur Jérôme J Bataillé-Simoneau Nelly N Campion Claire C Valot Benoît B Zivy Michel M Simoneau Philippe P Simoneau Philippe P Fillinger Sabine S

Proteomics 20140616 13-14

This study describes the gel-free phosphoproteomic analysis of the phytopathogenic fungi Alternaria brassicicola and Botrytis cinerea grown in vitro under nonlimiting conditions. Using a combination of strong cation exchange and IMAC prior to LC-MS, we identified over 1350 phosphopeptides per fungus representing over 800 phosphoproteins. The preferred phosphorylation sites were found on serine (>80%) and threonine (>15%), whereas phosphorylated tyrosine residues were found at less than 1% in A. ...[more]

PMID: 24825570

Similar Datasets

Project description:Background: Saprobic fungi are the predominant industrial sources of Carbohydrate Active enZymes (CAZymes) used for the saccharification of lignocellulose during the production of second generation biofuels. The production of more effective enzyme cocktails is a key objective for efficient biofuel production. To achieve this objective, it is crucial to understand the response of fungi to lignocellulose substrates. Our previous study used RNA-seq to identify the genes induced in Aspergillus niger in response to wheat straw, a biofuel feedstock, and showed that the range of genes induced was greater than previously seen with simple inducers [GSE33852]. Results: In this work we used RNA-seq to identify the genes induced in A. niger in response to short rotation coppice willow and compared this with the response to wheat straw from our previous study, at the same time-point. The response to willow showed a large increase in expression of genes encoding CAZymes. Genes encoding the major activities required to saccharify lignocellulose were induced on willow such as endoglucanases, cellobiohydrolases and xylanases. The transcriptome response to willow had many similarities with the response to straw with some significant differences in the expression levels of individual genes which are discussed in relation to differences in substrate composition or other factors. Differences in transcript levels include higher levels on wheat straw from genes encoding enzymes classified as members of GH62 (an arabinofuranosidase) and CE1 (a feruloyl esterase) CAZy families whereas two genes encoding endoglucanases classified as members of the GH5 family had higher transcript levels when exposed to willow. There were changes in the cocktail of enzymes secreted by A. niger when cultured with willow or straw. Assays for particular enzymes as well as saccharification assays were used to compare the enzyme activities of the cocktails. Wheat straw induced an enzyme cocktail that saccharified wheat straw to a greater extent than willow. Genes not encoding CAZymes were also induced on willow such as hydrophobins as well as genes of unknown function. Several genes were identified as promising targets for future study. Conclusions: By comparing this first study of the global transcriptional response of a fungus to willow with the response to straw, we have shown that the inducing lignocellulosic substrate has a marked effect upon the range of transcripts and enzymes expressed by A. niger. The use by industry of complex substrates such as wheat straw or willow could benefit efficient biofuel production. Six samples in total consisting of duplicate shake flask Aspergillus niger cultures from three conditions: glucose 48 h, willow 24 h, willow 24 h + glucose 5 h