Proteomics

Dataset Information

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ITRAQ-based quantitative proteomic analysis of membrane and nuclear fractions of glioma (WHO grade II, III and IV) tissues to map on Chromosome 12 and identification of mass spectrometry evidence for 'missing proteins'


ABSTRACT: Glioma tumors arise from normal glial cells (astrocytes). Gliomas are morphologically and clinically classified into four malignancy grades as Grade I, II, III and IV. Grade I and II comprise low grade gliomas that grow slowly, are well differentiated and have a better prognosis and survival compared to grades III and IV. In contrast, grade III-IV lack endothelial proliferation and are highly vascular with a tendency to infiltrate and have areas of extensive necrosis and hypoxia. In the present study, we used iTRAQ (isobaric tags for relative and absolute quantitation) - based quantitative proteomic approach followed by liquid chromatography and high resolution tandem mass spectrometry (LC-MS/MS) to identify differential membrane and nuclear proteins mapping to Chromosome 12 from three different grades of astrocytoma tumors (WHO Grade II, III and IV) compared to control.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

DISEASE(S): Brain Glioma

SUBMITTER: Ravi Sirdeshmukh  

LAB HEAD: Dr. Ravi Sirdeshmukh

PROVIDER: PXD000848 | Pride | 2019-06-04

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
GliomaGrIII_membrane.pep.xml Pepxml
GliomaGrIII_membrane.prot.xml Xml
GliomaGrIII_membrane_Chr12.mzData Other
GliomaGrIII_membrane_Chr12.mzML Mzml
GliomaGrII_membrane.pep.xml Pepxml
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Publications

Chromosome-centric human proteome project: deciphering proteins associated with glioma and neurodegenerative disorders on chromosome 12.

Gupta Manoj Kumar MK   Jayaram Savita S   Madugundu Anil K AK   Chavan Sandip S   Advani Jayshree J   Pandey Akhilesh A   Thongboonkerd Visith V   Sirdeshmukh Ravi R  

Journal of proteome research 20140624 7


In line with the aims of the Chromosome-centric Human Proteome Project (C-HPP) to completely annotate proteins of each chromosome and biology/disease driven HPP (B/D-HPP) to decipher their relation to diseases, we have generated a nonredundant catalogue of protein-coding genes for Chromosome 12 (Chr. 12) and further annotated proteins associated with major neurological disorders. Integrating high level proteomic evidence from four major databases (neXtProt, Global Proteome Machine (GPMdb), Pepti  ...[more]

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