Proteomics

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Cell type-resolved quantitative proteomics of mouse liver

ABSTRACT: Proteomics of pure cell populations provides a powerful approach to globally investigate biological function of individual cell types in mammalian organs. Here, we applied latest mass spectrometry techniques for in-depth proteomic analysis of freshly isolated hepatic cell types (HC-types) from murine liver. This study provides a valuable comprehensive resource for liver biology and biomedicine.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Hepatocyte, Liver, Permanent Cell Line Cell, Macrophage

SUBMITTER: Mario Oroshi  

LAB HEAD: Matthias Mann

PROVIDER: PXD000867 | Pride | 2014-10-20

REPOSITORIES: Pride

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20130408_EXQ6_BaAz_SA_H16R1_02.raw Raw
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Publications

Cell-type-resolved quantitative proteomics of murine liver.

Azimifar S Babak SB   Nagaraj Nagarjuna N   Cox Juergen J   Mann Matthias M  

Cell metabolism 20141201 6

Mass spectrometry (MS)-based proteomics provides a powerful approach to globally investigate the biological function of individual cell types in mammalian organs. Here, we applied this technology to the in-depth analysis of purified hepatic cell types from mouse. We quantified 11,520 proteins, making this the most comprehensive proteomic resource of any organ to date. Global protein copy number determination demonstrated that a large proportion of the hepatocyte proteome is dedicated to fatty ac  ...[more]