Proteomics

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Differences in proteasome targeting in Arabidopsis leaf mesophyll, epidermal and vascular cells

ABSTRACT: For the detailed and sensitive detection of the responses to a specific treatment it is important to perform tissue or cell type specific analyses, but this is not easily achievable for the different leaf tissues. Here we developed a method termed MeSelect to effectively separate leaf epidermis, vascular and mesophyll cells basically without contamination from other tissues. The high yield of the MeSelect method allowed for tissue specific high-throughput proteome analyses after inhibition of the proteasome with Syringolin A and the affinity enrichment of polyubiquitylated proteins in epidermis, mesophyll and vasculature.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Mesophyll, Leaf, Lower Epidermis

SUBMITTER: Katja Baerenfaller  

LAB HEAD: Katja Baerenfaller

PROVIDER: PXD000942 | Pride | 2015-05-13

REPOSITORIES: Pride

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Action DRS
20130219_15_Sep_1ex_urea1.RAW Raw
20130219_15_Sep_1ex_urea1.mgf Mgf
20130219_16_Sep_1ex_urea2.RAW Raw
20130219_16_Sep_1ex_urea2.mgf Mgf
20130219_17_Sep_1ex_urea3.RAW Raw
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Proteasome targeting of proteins in Arabidopsis leaf mesophyll, epidermal and vascular tissues.

Svozil Julia J   Gruissem Wilhelm W   Baerenfaller Katja K  

Frontiers in plant science 20150528

Protein and transcript levels are partly decoupled as a function of translation efficiency and protein degradation. Selective protein degradation via the Ubiquitin-26S proteasome system (UPS) ensures protein homeostasis and facilitates adjustment of protein abundance during changing environmental conditions. Since individual leaf tissues have specialized functions, their protein composition is different and hence also protein level regulation is expected to differ. To understand UPS function in  ...[more]

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