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The Pan-Human Library: A repository of assays to quantify 10 000 proteins by SWATH-MS

ABSTRACT: The “Pan-Human Library” is a compendium of highly specific assays covering more than 10 000 human proteins and enabling their targeted analysis in SWATH-MS datasets acquired from research or clinical specimens.

REANALYSED by: PAe005371PAe005368PAe005365PAe005353PAe005451PAe005450PAe005442PAe005436PAe005435PAe005426PAe005424PAe005423PAe005405PAe005403PAe005351PAe005347PAe005346PAe005337PAe005335PAe005327PAe005391PAe005389

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Large Intestine, Thp-1 Cell, Blood Plasma, Monocyte, Lung, U2-os Cell, Hek-293 Cell, Cal-51 Cell, Kidney, Hela Cell, Blood Platelet, Lncap Cell, Muscle, Neutrophil

SUBMITTER: George Rosenberger

LAB HEAD: Ruedi Aebersold

PROVIDER: PXD000953 | Pride | 2014-08-08

REPOSITORIES: Pride

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	ANTONV_J130605_003.mzXML	Mzxml
	ANTONV_J130605_004.mzXML	Mzxml
	ANTONV_J130605_005.mzXML	Mzxml
	ANTONV_J130605_006.mzXML	Mzxml
	ANTONV_J130605_007.mzXML	Mzxml

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A repository of assays to quantify 10,000 human proteins by SWATH-MS.

Rosenberger George G Koh Ching Chiek CC Guo Tiannan T Röst Hannes L HL Kouvonen Petri P Collins Ben C BC Heusel Moritz M Liu Yansheng Y Caron Etienne E Vichalkovski Anton A Faini Marco M Schubert Olga T OT Faridi Pouya P Ebhardt H Alexander HA Matondo Mariette M Lam Henry H Bader Samuel L SL Campbell David S DS Deutsch Eric W EW Moritz Robert L RL Tate Stephen S Aebersold Ruedi R

Scientific data 20140916

Mass spectrometry is the method of choice for deep and reliable exploration of the (human) proteome. Targeted mass spectrometry reliably detects and quantifies pre-determined sets of proteins in a complex biological matrix and is used in studies that rely on the quantitatively accurate and reproducible measurement of proteins across multiple samples. It requires the one-time, a priori generation of a specific measurement assay for each targeted protein. SWATH-MS is a mass spectrometric method th ...[more]

PMID: 25977788

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The Pan-Human Library: A repository of assays to quantify 10 000 proteins by SWATH-MS / SWATH-MS validation data

Project description:The “Pan-Human Library” is a compendium of highly specific assays covering more than 10 000 human proteins and enabling their targeted analysis in SWATH-MS datasets acquired from research or clinical specimens. This dataset contains validation SWATH-MS data and OpenSWATH results of whole cell lysates of HeLa (guot_L130330_005_SW, guot_L130330_006_SW, guot_L130330_007_SW) and U2OS cells (gout_L130330_013_SW, gout_L130330_014_SW, gout_L130330_015_SW). Further, the combined assay library (phl004_s32.csv) and the sample-specific assay libraries (phl004_sshela_s32.csv, phl004_ssu2os.csv) used for the analysis are provided.

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The Pan-Human Library: A repository of assays to quantify 10 000 proteins by SWATH-MS

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The Pan-Human Library: A repository of assays to quantify 10 000 proteins by SWATH-MS / SWATH-MS validation data

Project description:The â??Pan-Human Libraryâ?? is a compendium of highly specific assays covering more than 10 000 human proteins and enabling their targeted analysis in SWATH-MS datasets acquired from research or clinical specimens. This dataset contains validation SWATH-MS data and OpenSWATH results of whole cell lysates of HeLa (guot_L130330_005_SW, guot_L130330_006_SW, guot_L130330_007_SW) and U2OS cells (gout_L130330_013_SW, gout_L130330_014_SW, gout_L130330_015_SW). Further, the combined assay library (phl004_s32.csv) and the sample-specific assay libraries (phl004_sshela_s32.csv, phl004_ssu2os.csv) used for the analysis are provided.

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