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Proteomic analysis of human vitreous humor

ABSTRACT: In this study, we investigated the proteome of normal human vitreous humor using high resolution Fourier transform mass spectrometry. We identified 1269 proteins. This largest catalog of vitreous proteins to date should facilitate biomedical research into pathological conditions of the eye including diabetic retinopathy, retinal detachment and cataract.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Vitreous Humor

SUBMITTER: Akhilesh Pandey

LAB HEAD: Akhilesh Pandey

PROVIDER: PXD000957 | Pride | 2014-08-08

REPOSITORIES: Pride

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			Action	DRS
	VH1.raw	Raw
	VH10.raw	Raw
	VH10_results.2014_04_22_11_21_09.t.xml	Xml
	VH10_results_rev.2014_04_22_11_41_33.t.xml	Xml
	VH10r.raw	Raw

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Proteomic analysis of human vitreous humor.

Murthy Krishna R KR Goel Renu R Subbannayya Yashwanth Y Jacob Harrys Kc HK Murthy Praveen R PR Manda Srikanth Srinivas SS Patil Arun H AH Sharma Rakesh R Sahasrabuddhe Nandini A NA Parashar Arun A Nair Bipin G BG Krishna Venkatarangaiah V Prasad Ts Keshava TK Gowda Harsha H Pandey Akhilesh A

Clinical proteomics 20140714 1

<h4>Background</h4>The vitreous humor is a transparent, gelatinous mass whose main constituent is water. It plays an important role in providing metabolic nutrient requirements of the lens, coordinating eye growth and providing support to the retina. It is in close proximity to the retina and reflects many of the changes occurring in this tissue. The biochemical changes occurring in the vitreous could provide a better understanding about the pathophysiological processes that occur in vitreoretin ...[more]

PMID: 25097467

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Protein profile of vitreous from Reeler model

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Project description:INSC94Y transgenic pigs serve as a valuable model for permanent neonatal diabetes mellitus. Through genetically engineered modifications in the insulin (INS) gene, this model presents with impaired insulin secretion, resulting elevated fasting blood-glucose levels (hyperglycemia) [1].One of the severe complications of diabetes mellitus are hypoglycemia-mediated morphological abnormalities in the retina, also described as diabetic retinopathy (DR). Adjacent to the retina lies the vitreous, a gelatinous, highly hydrated matrix, which is important for ocular function and retinal physiology. It contains variety of proteins and signaling molecules, which are useful for the characterization of the biological activity of the vitreous itself, and may help to elucidate molecular processes occurring in the retina, especially under pathophysiological conditions and in disease. To gain insight into the proteomic profile of porcine vitreous and detect possible differences relevant to DR pathogenesis, we used discovery proteomics for analysis of INSC94Y vitreous compared to wild-type controls. Reference: [1] Renner, S.; Braun-Reichhart, C.; Blutke, A.; Herbach, N.; Emrich, D.; Streckel, E.; Wunsch, A.; Kessler, B.; Kurome, M.; Bahr, A.; et al. Permanent neonatal diabetes in INS(C94Y) transgenic pigs. Diabetes 2013, 62, 1505-1511, doi:10.2337/db12-1065.

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Proteomic Insight into the Role of Exosomes in Proliferative Vitreoretinopathy Development

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Protein composition of the subretinal fluid suggest selective diffusion of vitreous proteins in retinal detachment

Project description:Purpose: To study the proteome of the subretinal fluid (SRF) from rhegmatogenous retinal detachment (RRD) in search for novel markers for improved diagnosis and prognosis of RRD. Methods: Human undiluted SRF obtained during vitrectomy for primary RRD using a 41G needle (n = 24) was analyzed and compared to vitreous humor from 2-days post-mortem eyes (n = 20). Sample preparation underwent nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS). Label-free quantification (LFQ) using MaxQuant was used to determine differential expressed proteins between SRF and vitreous humor. The intensity-based absolute quantification (iBAQ) was used to rank proteins according to their molar fractions within groups. Identification of proteins beyond the quantitative level was performed using the Mascot search engine. Results: The protein concentration of the control vitreous humor was lower and more consistent (1.2 ± 0.4 mg) than that of the SRF (17.9 ± 22 mg). The iBAQ analysis showed high resemblance between SRF and vitreous humor, except for crystallins solely identified in vitreous humor. The LFQ analysis found 37 protein misregulations between SRF and vitreous humor of which the blood coagulation pathway was found to be enriched using the PANTHER Classification System. Combined, the iBAQ, LFQ and Mascot analysis found an overlap only in chitinase-3-like protein 1 and galectin-3-binding protein unique to the SRF. Conclusions: The proteome of the SRF was highly represented by proteins involved in proteolysis. Such proteins can possibly serve as targets in modulating the effects of SRF in RD.

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