Dataset Information

Proteomics of Alzheimer's Disease mouse models

ABSTRACT: Alzheimer’s disease (AD) is a neurodegenerative disease displaying plaques formed by the neurotoxic amyloid β-peptide (Aβ) and intracellular neurofibrillary tangles consisting of protein tau. However, how these pathologies relates to the massive neuronal death that occurs in AD brains remain elusive. To identify proteins, the levels of which are affected by increased Aβ levels, we performed a proteomic analysis of the AD mouse model APPsw and compared it to that of APPsw mice lacking the major Aβ degrading enzyme neprilysin. This was achieved by establishing an LC-MS/MS method to analyze brain homogenate, using an 18O-labeled internal standard to accurately quantify the protein levels. By this approach we identified approximately 600 proteins and could quantify the levels of 300 of these. Interestingly, several proteins, including some not previously reported to be associated with AD, were identified. The relevance of these proteins for AD warrants further research.

INSTRUMENT(S): LTQ

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

DISEASE(S): Alzheimer's Disease

SUBMITTER: Per Nilsson

LAB HEAD: Lars Tjernberg

PROVIDER: PXD000968 | Pride | 2015-07-29

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	120806_T_187_Nilsson_O18_01.RAW	Raw
	120806_T_187_Nilsson_O18_02.RAW	Raw
	120806_T_187_Nilsson_O18_03.RAW	Raw
	120807_T_187_Nilsson_O18_04.RAW	Raw
	120807_T_187_Nilsson_O18_05.RAW	Raw

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Publications

Loss of neprilysin alters protein expression in the brain of Alzheimer's disease model mice.

Nilsson Per P Loganathan Krishnapriya K Sekiguchi Misaki M Winblad Bengt B Iwata Nobuhisa N Saido Takaomi C TC Tjernberg Lars O LO

Proteomics 20150813 19

Alzheimer's disease (AD) is a neurodegenerative disease displaying extracellular plaques formed by the neurotoxic amyloid β-peptide (Aβ), and intracellular neurofibrillary tangles consisting of protein tau. However, how these pathologies relate to the massive neuronal death that occurs in AD brains remain elusive. Neprilysin is the major Aβ-degrading enzyme and a lack thereof increases Aβ levels in the brain twofold. To identify altered protein expression levels induced by increased Aβ levels, w ...[more]

PMID: 26194619

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