Proteomics

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Function of CK2-alpha in a minimal circadian system

ABSTRACT: Casein Kinase 2 (CK2) is an evolutionarily conserved kinase protein that phosphorylates a plethora of cellular target protein involved in processes including DNA repair, cell cycle control, and circadian rhythms. CK2 is functionally conserved across all eukaryotes, although the substrate proteins identified in targeted experiments on a diverse range of complex tissues are often different. A growing interest exists to identify conserved, core signalling principles essential to eukaryotic life. To efficiently study generic roles of CK2 in the cellular circadian clock, we used a minimal eukaryotic model organism (Ostreococcus tauri). Overexpression of CK2 leads to a slow circadian rhythm, verifying functional conservation of CK2 in cellular timekeeping. CK2 activity contributes more strongly to the clock around dusk than around dawn, and analysis of the phospho-proteome at this time revealed a strong overrepresentation of potential CK2 sites. Large changes to the phospho-proteome are reported here upon overexpression of CK2, identifying a set of CK2-responsive phospho-sites. To identify more CK2-specific phosphorylation events, the phospho-proteome of cells treated with CK2 inhibitor were analysed and compared to the overexpression lines. We thus report a full inventory of the CK2-responsive phospho-proteome to inform studies into CK2 activity in the circadian clock of more complex tissues, as science moves to understand and modulate cellular circadian rhythms to help us negate the negative effects of shiftwork or cross-continental travel.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Ostreococcus Tauri

TISSUE(S): Cell Suspension Culture, Bto:0000221, Cell Line Cell

DISEASE(S): Disease Free

SUBMITTER: Thierry Le Bihan  

LAB HEAD: Thierry Le bihan

PROVIDER: PXD000975 | Pride | 2015-08-11

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
120618_o1_02_so120612_ot_CCAI_2.RAW Raw
120618_o1_04_so120612_ot_CCAI_5.RAW Raw
120618_o1_05_so120612_ot_Ox21_5.RAW Raw
120618_o1_07_so120612_ot_Ox21_1.RAW Raw
120618_o1_08_so120612_ot_Ox21_4.RAW Raw
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Publications

Label-free quantitative analysis of the casein kinase 2-responsive phosphoproteome of the marine minimal model species Ostreococcus tauri.

Le Bihan Thierry T   Hindle Matthew M   Martin Sarah F SF   Barrios-Llerena Martin E ME   Krahmer Johanna J   Kis Katalin K   Millar Andrew J AJ   van Ooijen Gerben G  

Proteomics 20150609 23-24

Casein kinase 2 (CK2) is a protein kinase that phosphorylates a plethora of cellular target proteins involved in processes including DNA repair, cell cycle control, and circadian timekeeping. CK2 is functionally conserved across eukaryotes, although the substrate proteins identified in a range of complex tissues are often different. The marine alga Ostreococcus tauri is a unicellular eukaryotic model organism ideally suited to efficiently study generic roles of CK2 in the cellular circadian cloc  ...[more]